ArticleBiochemical pharmacology2012
The nicotinic acetylcholine receptors of zebrafish and an evaluation of pharmacological tools used for their study.
Article in Biochemical pharmacology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
27 citing papers in PubMed, 57 citations in OpenAlex.
- Nicotine and Cytisine Embryotoxicity in the Experimental Zebrafish Model.International journal of molecular sciences · 2023Article
- Behavioral Study of 3- and 5-Halocytisine Derivatives in Zebrafish Using the Novel Tank Diving Test (NTT).International journal of molecular sciences · 2023Article
- Allosteric inhibition of muscle-type nicotinic acetylcholine receptors by a neuromuscular blocking agent pancuronium.PloS one · 2023Article
- Chronic exposure to cigarette smoke extract increases nicotine withdrawal symptoms in adult and adolescent male rats.Advances in drug and alcohol research · 2023Article
- Advances in Zebrafish as a Comprehensive Model of Mental Disorders.Depression and anxiety · 2023Review
- Zebrafish Larvae Behavior Models as a Tool for Drug Screenings and Pre-Clinical Trials: A Review.International journal of molecular sciences · 2022Review
- Differentiating the Neuropharmacological Properties of Nicotinic Acetylcholine Receptor-Activating Alkaloids.Frontiers in pharmacology · 2022Article
- Conservation of mechanisms regulating emotional-like responses on spontaneous nicotine withdrawal in zebrafish and mammals.Progress in neuro-psychopharmacology & biological psychiatry · 2021Article
- Exploring Neurobehaviour in Zebrafish Embryos as a Screening Model for Addictiveness of Substances.Toxics · 2021Article
- Effects of nicotinic acetylcholine receptor-activating alkaloids on anxiety-like behavior in zebrafish.Journal of natural medicines · 2021Article
- Effects of Natural Monoamine Oxidase Inhibitors on Anxiety-Like Behavior in Zebrafish.Frontiers in pharmacology · 2021Article
- Sinomenine Hydrochloride Ameliorates Fish Foodborne EnteritisFrontiers in immunology · 2021Article
- Identification ofeLife · 2020Article
- Assessing the hazard of E-Cigarette flavor mixtures using zebrafish.Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association · 2020Article
- Neurotoxicity of organophosphate pesticides could reduce the ability of fish to escape predation under low doses of exposure.Scientific reports · 2019Article
- Zebrafish Get Connected: Investigating Neurotransmission Targets and Alterations in Chemical Toxicity.Toxics · 2016Article
- A critical review of neonicotinoid insecticides for developmental neurotoxicity.Critical reviews in toxicology · 2016Review
- Zebrafish mutants of the neuromuscular junction: swimming in the gene pool.The journal of physiological sciences : JPS · 2015Review
- Motoneuron axon pathfinding errors in zebrafish: differential effects related to concentration and timing of nicotine exposure.Toxicology and applied pharmacology · 2015Article
- Molecular psychiatry of zebrafish.Molecular psychiatry · 2015Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 1 country.
Funding
Abstract
Zebrafish (Danio rerio) have been used to study multiple effects of nicotine, for example on cognition, locomotion, and stress responses, relying on the assumption that pharmacological tools will operate similarly upon molecular substrates in the fish and mammalian systems. We have cloned the zebrafish nicotinic acetylcholine receptor (nAChR) subunits and expressed key nAChR subtypes in Xenopus oocytes including neuronal (α4β2, α2β2, α3β4, and α7) and muscle (α1β1(b)ɛδ) nAChR. Consistent with studies of mammalian nAChR, nicotine was relatively inactive on muscle-type receptors, having both low potency and efficacy. It had high efficacy but low potency for α7 receptors, and the best potency and good efficacy for α4β2 receptors. Cytisine, a key lead compound for the development of smoking cessation agents, is a full agonist for both mammalian α7 and α3β4 receptors, but a full agonist only for the fish α7, with surprisingly low efficacy for α3β4. The efficacy of cytisine for α4β2 was somewhat greater than typically reported for mammalian α4β2. The ganglionic blocker mecamylamine was most potent for blocking α3β4 receptors, least potent for α7, and roughly equipotent for the muscle receptors and the β2-containing nAChR. However, the block of β2-containing receptors was slowly reversible, consistent with effective targeting of these CNS-type receptors in vivo. Three prototypical α7-selective agonists, choline, tropane, and 4OH-GTS-21, were tested, and these agents were observed to activate both fish α7 and α4β2 nAChR. Our data therefore indicate that while some pharmacological tools used in zebrafish may function as expected, others will not.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.