ArticleMolecular and cellular neurosciences2012
Dynamic expression of ganglion cell markers in retinal progenitors during the terminal cell cycle.
Article in Molecular and cellular neurosciences, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 36 citations in OpenAlex.
- Article
- Intrinsic and Induced Neuronal Regeneration in the Mammalian Retina.Antioxidants & redox signaling · 2023Review
- Notch directs telencephalic development and controls neocortical neuron fate determination by regulating microRNA levels.Development (Cambridge, England) · 2023Article
- Lhx2 is a progenitor-intrinsic modulator of Sonic Hedgehog signaling during early retinal neurogenesis.eLife · 2022Article
- The autism-associated Meis2 gene is necessary for cardiac baroreflex regulation in mice.Scientific reports · 2022Article
- An enigmatic translocation of the vertebrate primordial eye field.BMC evolutionary biology · 2020Review
- TheProceedings of the National Academy of Sciences of the United States of America · 2020Article
- A Novel Reporter Mouse Uncovers Endogenous Brn3b Expression.International journal of molecular sciences · 2019Article
- Differentiation of retinal ganglion cells from induced pluripotent stem cells: a review.International journal of ophthalmology · 2019Review
- Islet1 and Brn3 Expression Pattern Study in Human Retina and hiPSC-Derived Retinal Organoid.Stem cells international · 2019Article
- The multisystemic functions of FOXD1 in development and disease.Journal of molecular medicine (Berlin, Germany) · 2018Review
- Different Effect ofFrontiers in genetics · 2018Article
- VEGF-A and neuropilin 1 (NRP1) shape axon projections in the developing CNS via dual roles in neurons and blood vessels.Development (Cambridge, England) · 2017Article
- Uncoupling of neurogenesis and differentiation during retinal development.The EMBO journal · 2017Article
- Prenatal ethanol exposure in mice phenocopies Cdon mutation by impeding Shh function in the etiology of optic nerve hypoplasia.Disease models & mechanisms · 2017Article
- Characterization of Three-Dimensional Retinal Tissue Derived from Human Embryonic Stem Cells in Adherent Monolayer Cultures.Stem cells and development · 2015Article
- Two transcription factors, Pou4f2 and Isl1, are sufficient to specify the retinal ganglion cell fate.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Cavefish eye loss in response to an early block in retinal differentiation progression.Development (Cambridge, England) · 2015Article
- Differential responsiveness of distinct retinal domains to Atoh7.Mechanisms of development · 2014Article
- Connecting the retina to the brain.ASN neuro · 2014Review
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
The vertebrate neural retina contains seven major cell types, which arise from a common multipotent progenitor pool. During neurogenesis, these cells stop cycling, commit to a single fate, and differentiate. The mechanism and order of these steps remain unclear. The first-born type of retinal neurons, ganglion cells (RGCs), develop through the actions of Math5 (Atoh7), Brn3b (Pou4f2) and Islet1 (Isl1) factors, whereas inhibitory amacrine and horizontal precursors require Ptf1a for differentiation. We have examined the link between these markers, and the timing of their expression during the terminal cell cycle, by nucleoside pulse-chase analysis in the mouse retina. We show that G2 phase lasts 1-2 h at embryonic (E) 13.5 and E15.5 stages. Surprisingly, we found that cells expressing Brn3b and/or Isl1 were frequently co-labeled with EdU after a short chase (<1 h) in early embryos (<E14), indicating that these factors, which mark committed RGCs, can be expressed during S or G2 phases. However, during late development (>E15), Brn3b and Isl1 were exclusively expressed in post-mitotic cells, even as new RGCs are still generated. In contrast, Ptf1a and amacrine marker AP2α were detected only after terminal mitosis, at all developmental stages. Using a retroviral tracer in embryonic retinal explants (E12-E13), we identified two-cell clones containing paired ganglion cells, consistent with RGC fate commitment prior to terminal mitosis. Thus, although cell cycle exit and fate determination are temporally correlated during retinal neurogenesis, the order of these events varies according to developmental stage and final cell type.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.