ArticleStructure (London, England : 1993)2012
Interaction of the mediator head module with RNA polymerase II.
Article in Structure (London, England : 1993), 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 33 citations in OpenAlex.
- Architectural Mediator subunits are differentially essential for global transcription in Saccharomyces cerevisiae.Genetics · 2021Article
- Twenty years of Mediator complex structural studies.Biochemical Society transactions · 2019Review
- Structural Insights into the Eukaryotic Transcription Initiation Machinery.Annual review of biophysics · 2017Review
- PIC Activation through Functional Interplay between Mediator and TFIIH.Journal of molecular biology · 2017Article
- Role for the MED21-MED7 Hinge in Assembly of the Mediator-RNA Polymerase II Holoenzyme.The Journal of biological chemistry · 2016Article
- Article
- Structural basis of transcription initiation by RNA polymerase II.Nature reviews. Molecular cell biology · 2015Review
- Article
- Reconstitution of active human core Mediator complex reveals a critical role of the MED14 subunit.Nature structural & molecular biology · 2014Article
- Disordered proteinaceous machines.Chemical reviews · 2014Review
- Redefining the modular organization of the core Mediator complex.Cell research · 2014Article
- Subunit architecture and functional modular rearrangements of the transcriptional mediator complex.Cell · 2014Article
- An evolving understanding of nuclear receptor coregulator proteins.Journal of molecular endocrinology · 2013Review
- Structural flexibility and functional interaction of Mediator Cdk8 module.Protein & cell · 2013Article
- The head module of Mediator directs activation of preloaded RNAPII in vivo.Nucleic acids research · 2013Article
- Mediator is an intrinsic component of the basal RNA polymerase II machinery in vivo.Nucleic acids research · 2013Article
- Correct assembly of RNA polymerase II depends on the foot domain and is required for multiple steps of transcription in Saccharomyces cerevisiae.Molecular and cellular biology · 2013Article
- A conserved Mediator-CDK8 kinase module association regulates Mediator-RNA polymerase II interaction.Nature structural & molecular biology · 2013Article
- Srb5/Med18-mediated termination of transcription is dependent on gene looping.The Journal of biological chemistry · 2013Article
- The Mediator complex and transcription elongation.Biochimica et biophysica acta · 2013Review
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Authors and funding
8 authors at 4 institutions in 1 country.
Funding
Abstract
Mediator, a large (21 polypeptides, MW ∼1 MDa) complex conserved throughout eukaryotes, plays an essential role in control of gene expression by conveying regulatory signals that influence the activity of the preinitiation complex. However, the precise mode of interaction between Mediator and RNA polymerase II (RNAPII), and the mechanism of regulation by Mediator remain elusive. We used cryo-electron microscopy and reconstituted in vitro transcription assays to characterize a transcriptionally-active complex including the Mediator Head module and components of a minimum preinitiation complex (RNAPII, TFIIF, TFIIB, TBP, and promoter DNA). Our results reveal how the Head interacts with RNAPII, affecting its conformation and function.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.