ArticleThe Journal of allergy and clinical immunology2012
Role of nicotinic receptors and acetylcholine in mucous cell metaplasia, hyperplasia, and airway mucus formation in vitro and in vivo.
Article in The Journal of allergy and clinical immunology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers, 1 of them a synthesis that pooled it.
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Who cites it
26 citing papers in PubMed, 1 synthesis or guideline pooled it, 45 citations in OpenAlex.
- Smoking and vaping alter genes related to mechanisms of SARS-CoV-2 susceptibility and severity: a systematic review and meta-analysis.The European respiratory journal · 2024Pooled it
- The effects of e-cigarette use on asthma severity in adult BALB/c mice.Experimental physiology · 2026Article
- Article
- α7nAChR Activation Combined with Endothelial Progenitor Cell Transplantation Attenuates Lung Injury in Diabetic Rats with Sepsis through the NF-κB Pathway.Inflammation · 2024Article
- Enhancement of airway ciliary beating mediated via voltage-gated CaPflugers Archiv : European journal of physiology · 2022Article
- Sulphide donors affect the expression of mucin and sulphide detoxification genes in the mucosal organs of Atlantic salmon (Frontiers in physiology · 2022Article
- Differential responses to e-cig generated aerosols from humectants and different forms of nicotine in epithelial cells from nonsmokers and smokers.American journal of physiology. Lung cellular and molecular physiology · 2021Article
- COVID-19 and COPD: a narrative review of the basic science and clinical outcomes.European respiratory review : an official journal of the European Respiratory Society · 2020Review
- Evaluation of secondhand smoke effects on CFTR function in vivo.Respiratory research · 2020Article
- Acetylcholinesterase Inhibitor Pyridostigmine Bromide Attenuates Gut Pathology and Bacterial Dysbiosis in a Murine Model of Ulcerative Colitis.Digestive diseases and sciences · 2020Article
- The Interplay Between Respiratory Microbiota and Innate Immunity in Flavor E-Cigarette Vaping Induced Lung Dysfunction.Frontiers in microbiology · 2020Review
- Dual Substance Use of Electronic Cigarettes and Alcohol.Frontiers in physiology · 2020Review
- Cigarette smoke and HIV synergistically affect lung pathology in cynomolgus macaques.The Journal of clinical investigation · 2018Article
- A Novel Orally Available Asthma Drug Candidate That Reduces Smooth Muscle Constriction and Inflammation by Targeting GABAMolecular pharmaceutics · 2018Article
- The investigation of effect of alpha lipoic acid against damage on neonatal rat lung to maternal tobacco smoke exposure.Toxicology reports · 2018Article
- Recent updates on electronic cigarette aerosol and inhaled nicotine effects on periodontal and pulmonary tissues.Oral diseases · 2017Review
- Electronic Cigarettes: Their Constituents and Potential Links to Asthma.Current allergy and asthma reports · 2017Review
- Optimization of substituted imidazobenzodiazepines as novel asthma treatments.European journal of medicinal chemistry · 2017Article
- Lung epithelial response to cigarette smoke and modulation by the nicotinic alpha 7 receptor.PloS one · 2017Article
- Chronic electronic cigarette exposure in mice induces features of COPD in a nicotine-dependent manner.Thorax · 2016Article
Corrections and comments
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Authors and funding
14 authors at 4 institutions in 1 country.
Funding
Abstract
backgroundAirway mucus hypersecretion is a key pathophysiologic feature in a number of lung diseases. Cigarette smoke/nicotine and allergens are strong stimulators of airway mucus; however, the mechanism of mucus modulation is unclear.
objectivesWe sought to characterize the pathway by which cigarette smoke/nicotine regulates airway mucus and identify agents that decrease airway mucus.
methodsIL-13 and γ-aminobutyric acid type A receptors (GABA(A)Rs) are implicated in airway mucus. We examined the role of IL-13 and GABA(A)Rs in nicotine-induced mucus formation in normal human bronchial epithelial (NHBE) and A549 cells and secondhand cigarette smoke-induced, ovalbumin-induced, or both mucus formation in vivo.
resultsNicotine promotes mucus formation in NHBE cells; however, the nicotine-induced mucus formation is independent of IL-13 but sensitive to the GABA(A)R antagonist picrotoxin. Airway epithelial cells express α7-, α9-, and α10-nicotinic acetylcholine receptors (nAChRs), and specific inhibition or knockdown of α7- but not α9/α10-nAChRs abrogates mucus formation in response to nicotine and IL-13. Moreover, addition of acetylcholine or inhibition of its degradation increases mucus in NHBE cells. Nicotinic but not muscarinic receptor antagonists block allergen- or nicotine/cigarette smoke-induced airway mucus formation in NHBE cells, murine airways, or both.
conclusionsNicotine-induced airway mucus formation is independent of IL-13, and α7-nAChRs are critical in airway mucous cell metaplasia/hyperplasia and mucus production in response to various promucoid agents, including IL-13. In the absence of nicotine, acetylcholine might be the biological ligand for α7-nAChRs to trigger airway mucus formation. α7-nAChRs are downstream of IL-13 but upstream of GABA(A)Rα2 in the MUC5AC pathway. Acetylcholine and α7-nAChRs might serve as therapeutic targets to control airway mucus.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.