ArticleCurrent eye research2012
CFTR-mediated Cl(-) transport in the acinar and duct cells of rabbit lacrimal gland.
Article in Current eye research, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed, 12 citations in OpenAlex.
- Membrane-associated mucins of the ocular surface: New genes, new protein functions and new biological roles in human and mouse.Progress in retinal and eye research · 2020Review
- The regulatory role of vasoactive intestinal peptide in lacrimal gland ductal fluid secretion: A new piece of the puzzle in tear production.Molecular vision · 2020Article
- Novel Insight Into the Role of CFTR in Lacrimal Gland Duct Function in Mice.Investigative ophthalmology & visual science · 2018Article
- Characterization of Na+-K+-2Cl- Cotransporter Activity in Rabbit Lacrimal Gland Duct Cells.Investigative ophthalmology & visual science · 2016Article
- Small-molecule CFTR activators increase tear secretion and prevent experimental dry eye disease.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2016Article
- ENaC in the Rabbit Lacrimal Gland and its Changes During Sjögren Syndrome and Pregnancy.Eye & contact lens · 2015Article
- Revisiting CFTR inhibition: a comparative study of CFTRinh -172 and GlyH-101 inhibitors.British journal of pharmacology · 2014Article
- Lacrimal glands in cystic fibrosis.Saudi journal of ophthalmology : official journal of the Saudi Ophthalmological Society · 2013Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
purposeWe investigated the role that the cystic fibrosis transmembrane conductance regulator (CFTR) may play in Cl(-) transport in the acinar and ductal epithelial cells of rabbit lacrimal gland (LG).
methodsPrimary cultured LG acinar cells were processed for whole-cell patch-clamp electrophysiological recording of Cl(-) currents by using perfusion media with high and low [Cl(-)], 10 µM forskolin and 100 µM 3-isobutyl-1-methylxanthine (IBMX), the non-specific Cl(-) channel blocker 4,4'-disothiocyanostilbene-2, 2' sulphonic acid (DIDS; 100 µM) and CFTRinh-172 (10 µM), a specific blocker for CFTR. Ex vivo live cell imaging of [Cl(-)] changes in duct cells was performed on freshly dissected LG duct with a multiphoton confocal laser scanning microscope using a Cl(-) sensitive fluorescence dye, N-[ethoxycarbonylmethyl]-6-methoxy-quinolinium bromide.
resultsWhole-cell patch-clamp studies demonstrated the presence of Cl(-) current in isolated acinar cells and revealed that this Cl(-) current was mediated by CFTR channel. Live cell imaging also showed the presence of CFTR-mediated Cl(-) transport across the plasma membrane of duct cells.
conclusionsOur previous data showed the presence of CFTR in all acinar and duct cells within the rabbit LG, with expression most prominent in the apical membranes of duct cells. The present study demonstrates that CFTR is actively involved in Cl(-) transport in both acinar cells and epithelial cells from duct segments, suggesting that CFTR may play a significant role in LG secretion.
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