ArticleBioTechniques2012
Simplified and versatile method for isolation of high-quality RNA from pancreas.
Article in BioTechniques, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 33 citations in OpenAlex.
- Improved stabilisation of human pancreas biopsies: Comparative evaluation of RNA preservation methods.World journal of biological chemistry · 2026Article
- Comparative transcriptomics reveal the common anteroposterior molecular blueprint of adult bilaterian guts.PLoS biology · 2026Article
- Establishing and Validating a Biomolecular Signature of Ischemia/Reperfusion Injury in a Porcine Pancreas Allotransplantation Model.Transplantation direct · 2025Article
- Article
- TBX3 is dynamically expressed in pancreatic organogenesis and fine-tunes regeneration.BMC biology · 2023Article
- Spatial transcriptomics using multiplexed deterministic barcoding in tissue.Nature communications · 2023Article
- Article
- MyD88 deficiency aggravates the severity of acute pancreatitis by promoting MyD88-independent TRIF pathway-mediated necrosis.Annals of translational medicine · 2022Article
- Article
- Chronic jetlag-induced alterations in pancreatic diurnal gene expression.Physiological genomics · 2021Article
- Method Optimization for Extracting High-Quality RNA From the Human Pancreas Tissue.Translational oncology · 2018Article
- Article
- Characterization of RNA isolated from eighteen different human tissues: results from a rapid human autopsy program.Cell and tissue banking · 2016Article
- Type 1 diabetes cadaveric human pancreata exhibit a unique exocrine tissue proteomic profile.Proteomics · 2016Article
- Preservation of Multiple Mammalian Tissues to Maximize Science Return from Ground Based and Spaceflight Experiments.PloS one · 2016Article
- Hepatic transcriptome implications for palm fruit juice deterrence of type 2 diabetes mellitus in young male Nile rats.Genes & nutrition · 2016Article
- RNA isolation from mouse pancreas: a ribonuclease-rich tissue.Journal of visualized experiments : JoVE · 2014Article
- Optimization of RNA extraction from rat pancreatic tissue.Iranian journal of medical sciences · 2014Article
- Comparative analysis of RNA sequencing methods for degraded or low-input samples.Nature methods · 2013Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Isolation of high-quality RNA from pancreas is challenging because the organ contains large quantities of RNases and undergoes autolysis upon harvest. Here we present a simplified perfusion method of the pancreas using an RNase inhibitor. The technique consistently yields high-quality RNA from stored pancreas samples suitable for molecular biology applications, including quantitative RT-PCR. Yields are comparable to RNA isolated from pancreas immediately, but superior to RNA isolated from stored samples that were snap-frozen or immersed in an RNase inhibitor solution. In addition, when compared to the previously reported in situ ductal perfusion technique, our method does not cause histological artifacts.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.