ArticleExperimental diabetes research2012
The biological behaviors of rat dermal fibroblasts can be inhibited by high levels of MMP9.
Article in Experimental diabetes research, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed, 28 citations in OpenAlex.
- A bibliometric and visual analysis of colorectal cancer-diabetes comorbidity.Discover oncology · 2026Article
- Targeting matrix metalloproteases in diabetic wound healing.Frontiers in immunology · 2023Review
- Heme oxygenase-1 alleviates advanced glycation end product-induced oxidative stress, inflammatory response and biological behavioral disorders in rat dermal fibroblasts.Experimental and therapeutic medicine · 2021Article
- Assessment of Selected Matrix Metalloproteinases (MMPs) and Correlation with Cytokines in Psoriatic Patients.Mediators of inflammation · 2021Article
- The molecular biology in wound healing & non-healing wound.Chinese journal of traumatology = Zhonghua chuang shang za zhi · 2017Review
- Clinical Relevance of NGAL/MMP-9 Pathway in Patients with Endometrial Cancer.Disease markers · 2017Article
- Low-Dose Homocystine Enhances Proliferation and Migration of Bv2 Microglia Cells.Cellular and molecular neurobiology · 2016Article
- Glucose toxic effects on granulation tissue productive cells: the diabetics' impaired healing.BioMed research international · 2013Review
- Gene expression profiling of early hepatic stellate cell activation reveals a role for Igfbp3 in cell migration.PloS one · 2013Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
aimsTo explore the effects of the high expression of MMP9 on biological behaviors of fibroblasts.
methodsHigh glucose and hyperhomocysteine were used to induce MMP9 expression in skin fibroblasts. Cell proliferation was detected by flow cytometry and cell viability by CCK-8. ELISA assay was used to detect collagen (hydroxyproline) secretion. Scratch test was employed to evaluate horizontal migration of cells and transwell method to evaluate vertical migration of cells.
resultsThe mRNA and protein expressions of MMP9 and its protease activity were significantly higher in cells treated with high glucose and hyperhomocysteine than those in control group. At the same time, the S-phase cell ratio, proliferation index, cell viability, collagen (hydroxyproline) secretion, horizontal migration rate, and the number of vertical migration cells decreased in high-glucose and hyperhomocysteine-treated group. Tissue inhibitor of metalloproteinase 1 (TIMP1), which inhibits the activity of MMP9, recovered the above biological behaviors.
conclusionsHigh expression of MMP9 in skin fibroblasts could be induced by cultureing in high glucose and hyperhomocysteine medium, which inhibited cell biological behaviors. Inhibitions could be reversed by TIMP1. The findings suggested that MMP9 deters the healing of diabetic foot ulcers by inhibiting the biological behaviors of fibroblasts.
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