ArticleJournal of nuclear medicine : official publication, Society of Nuclear Medicine2012
PET with 64Cu-histidine for noninvasive diagnosis of biliary copper excretion in Long-Evans cinnamon rat model of Wilson disease.
Article in Journal of nuclear medicine : official publication, Society of Nuclear Medicine, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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12 citing papers in PubMed, 16 citations in OpenAlex.
- Novel PSCA-Targeting Adapter Molecules for Late-Stage RevCAR-T Cell Therapy in Prostate Cancer.International journal of molecular sciences · 2026Article
- High value ofMolecular therapy. Methods & clinical development · 2022Article
- Non-invasive radionuclide imaging of trace metal trafficking in health and disease: "PET metallomics".RSC chemical biology · 2022Review
- Insights into Trace Metal Metabolism in Health and Disease from PET: "PET Metallomics".Journal of nuclear medicine : official publication, Society of Nuclear Medicine · 2018Review
- Animal models of Wilson disease.Journal of neurochemistry · 2018Review
- Copper-64: a real theranostic agent.Drug design, development and therapy · 2018Review
- Studies of copper trafficking in a mouse model of Alzheimer's disease by positron emission tomography: comparison ofMetallomics : integrated biometal science · 2017Article
- Demonstrating Potential of Cell Therapy for Wilson's Disease with the Long-Evans Cinnamon Rat Model.Methods in molecular biology (Clifton, N.J.) · 2017Article
- Cell therapy to remove excess copper in Wilson's disease.Annals of the New York Academy of Sciences · 2014Review
- Positron emission tomography for measurement of copper fluxes in live organisms.Annals of the New York Academy of Sciences · 2014Article
- Diagnosis of abnormal biliary copper excretion by positron emission tomography with targeting of (64)Copper-asialofetuin complex in LEC rat model of Wilson's disease.American journal of nuclear medicine and molecular imaging · 2014Article
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6 authors at 4 institutions in 1 country.
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Abstract
unlabelledExcretion of copper into bile requires the copper transporter Atp7b, which is deficient in Wilson disease. We hypothesized that a radiocopper-histidine complex would be effective for diagnosing Wilson disease by molecular imaging and tested this hypothesis in the Long-Evans cinnamon (LEC) rat model with Atp7b deficiency.
methodsWe complexed (64)Cu to l-histidine and analyzed clearance from blood, uptake in tissues, and excretion in bile of healthy Long-Evans agouti (LEA) rats versus LEC rats modeling Wilson disease. Sixty-minute dynamic PET recordings were obtained in LEA and LEC rats. Possible effects of acute and chronic liver injury induced by carbon tetrachloride were studied in LEA rats. Atp7b deficiency in LEC rats was reconstituted by transplantation of healthy cells to establish the specificity of findings.
resultsExamination of blood, tissue, and bile showed that in healthy rats, radiocopper was incorporated in the liver, followed by rapid excretion in bile. Corresponding blood, tissue, and bile studies in LEC rats showed incorporation of radiocopper in the liver but without copper excretion in bile, leading to hepatic retention of the radiotracer. PET showed onset of copper clearance in the liver of LEA rats, whereas liver copper content progressively increased in LEC rats during the 1-h period. Hepatic radiocopper excretion was not altered by either acute or chronic liver injury. In LEC rats with liver repopulation by transplanted healthy hepatocytes, excretion of radiocopper confirmed that Atp7b was responsible for this effect.
conclusionImaging with the radiocopper-histidine complex successfully identified Atp7b-dependent biliary copper excretion. This principle will advance molecular imaging for Wilson disease.
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