ArticleNature protocols2012
Visualization of gene expression in whole mouse retina by in situ hybridization.
Article in Nature protocols, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
21 citing papers in PubMed.
- A Novel Retinal Nerve Fiber Layer Biomarker of Amyotrophic Lateral Sclerosis (ALS) Identified Using Longitudinal in vivo Ocular Imaging.Eye and brain · 2025Article
- Protocol for single-molecule FISH in the developing mouse retinal vasculature.STAR protocols · 2024Article
- Intravitreal Injection of AAV for the Transduction of Mouse Retinal Ganglion Cells.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Cre toxicity in mouse models of cardiovascular physiology and disease.Nature cardiovascular research · 2022Article
- Intersectional, anterograde transsynaptic targeting of neurons receiving monosynaptic inputs from two upstream regions.Communications biology · 2022Article
- Pericyte Bridges in Homeostasis and Hyperglycemia.Diabetes · 2020Article
- Caspase-8 modulates physiological and pathological angiogenesis during retina development.The Journal of clinical investigation · 2019Article
- Molecular signatures of retinal ganglion cells revealed through single cell profiling.Scientific reports · 2019Article
- Article
- Developmental SMAD6 loss leads to blood vessel hemorrhage and disrupted endothelial cell junctions.Developmental biology · 2018Article
- Dissection and Whole Mount Staining of Retina from Neonatal Mice.Bio-protocol · 2018Article
- Consensus guidelines for the use and interpretation of angiogenesis assays.Angiogenesis · 2018Review
- Dexamethasone-Mediated Upregulation of Calreticulin Inhibits Primary Human Glioblastoma Dispersal Ex Vivo.International journal of molecular sciences · 2018Article
- PDGFRβ-P2A-CreERAngiogenesis · 2017Article
- Plastic roles of pericytes in the blood-retinal barrier.Nature communications · 2017Article
- O-GlcNAc on NOTCH1 EGF repeats regulates ligand-induced Notch signaling and vascular development in mammals.eLife · 2017Article
- A long non-coding RNA interacts with Gfra1 and maintains survival of mouse spermatogonial stem cells.Cell death & disease · 2016Article
- Macrophages: An Inflammatory Link Between Angiogenesis and Lymphangiogenesis.Microcirculation (New York, N.Y. : 1994) · 2016Review
- Three-dimensional spheroid-cultured mesenchymal stem cells devoid of embolism attenuate brain stroke injury after intra-arterial injection.Stem cells and development · 2014Article
- The size of mesenchymal stem cells is a significant cause of vascular obstructions and stroke.Stem cell reviews and reports · 2014Article
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
The mouse retinal vasculature provides a powerful model system for studying development and pathologies of the vasculature. Because it forms as a two-dimensional flat plexus, it is easily imaged in its entirety in whole-mount retinal preparations. In order to study molecular signaling mechanisms, it is useful to visualize the expression of specific genes in the entire vascular plexus and retina. However, in situ hybridization on whole-mount retinal preparations is problematic because isolated retinas have a tendency to curl up during hybridization and are difficult to stain. Here we provide a detailed protocol that overcomes these difficulties and visualizes the mRNA distribution of one or two genes in the context of the counterstained retinal vasculature. The protocol takes 3-4 d for single-probe stains, with an additional 2 d for immunohistochemistry co-labeling. In situ hybridization with two probes adds a further 3 d.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.