ArticleJournal of molecular and cellular cardiology2012
Automated imaging reveals a concentration dependent delay in reversibility of cardiac myocyte hypertrophy.
Article in Journal of molecular and cellular cardiology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed.
- Cardiac unloading models for myocardial reverse remodeling.Basic research in cardiology · 2026Review
- Single-cell dynamics reveal a stress-induced decision between hypertrophy and apoptosis in neonatal rat cardiomyocytes.Journal of molecular and cellular cardiology plus · 2025Article
- Subcellular activation of β-adrenergic receptors using a spatially restricted antagonist.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Increased length-dependent activation of human engineered heart tissue after chronic αAmerican journal of physiology. Heart and circulatory physiology · 2023Article
- Investigating β-adrenergic-induced cardiac hypertrophy through computational approach: classical and non-classical pathways.The journal of physiological sciences : JPS · 2018Article
- Endothelin-1 promotes hypertrophic remodelling of cardiac myocytes by activating sustained signalling and transcription downstream of endothelin type A receptors.Cellular signalling · 2017Article
- Hypertrophy changes 3D shape of hiPSC-cardiomyocytes: Implications for cellular maturation in regenerative medicine.Cellular and molecular bioengineering · 2017Article
- Unraveling the complexities of cardiac remodeling and hypertrophy - high-content screening and computational modeling.Journal of molecular and cellular cardiology · 2014Article
- Phenotypic screen quantifying differential regulation of cardiac myocyte hypertrophy identifies CITED4 regulation of myocyte elongation.Journal of molecular and cellular cardiology · 2014Article
- Increasing the Content of High-Content Screening: An Overview.Journal of biomolecular screening · 2014Review
- PKA catalytic subunit compartmentation regulates contractile and hypertrophic responses to β-adrenergic signaling.Journal of molecular and cellular cardiology · 2014Article
- Intracrine endothelin signaling evokes IP3-dependent increases in nucleoplasmic Ca²⁺ in adult cardiac myocytes.Journal of molecular and cellular cardiology · 2013Article
- Network reconstruction and systems analysis of cardiac myocyte hypertrophy signaling.The Journal of biological chemistry · 2012Article
Corrections and comments
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Authors and funding
2 authors.
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Abstract
Cardiac hypertrophy is controlled by a dense signaling network with many pathways associated with cardiac myocyte growth. New large scale methodology is required to quantitatively characterize the pathways that distinguish reversible forms of hypertrophy from irreversible forms that lead to heart failure. Our automated image acquisition method records 5×5 mosaic images of fluorescent protein-labeled cardiac myocytes within each well of a 96-well plate using an automated stage and focus. Post-processing algorithms automatically identify cell edges, quantify cell phenotypes, and track cells. We uniquely applied our imaging platform to study hypertrophy reversibility in a scalable cell model. Cell area changes after washout of a dose response to the α-adrenergic receptor (αAR) agonist phenylephrine (PE) showed that hypertrophy reverses at low but not high levels of α-adrenergic signaling: a reversibility delay. Perturbations with specialized αAR antagonists, a mathematical model, and live imaging of αAR localization identify the mechanism for this reversibility delay: ligand trapping with internalized PE acting on intracellular αAR's.
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