ArticleThe journal of physical chemistry. B2012
High-throughput fluorescence-activated nanoscale subcellular sorter with single-molecule sensitivity.
Article in The journal of physical chemistry. B, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
6 citing papers in PubMed.
- Sizing Extracellular Vesicles Using Membrane Dyes and a Single Molecule-Sensitive Flow Analyzer.Analytical chemistry · 2021Article
- Micro- and Nano-Devices for Studying Subcellular Biology.Small (Weinheim an der Bergstrasse, Germany) · 2021Review
- Mitochondrial isolation: when size matters.Wellcome open research · 2020Review
- Back-focal-plane interferometric detection of nanoparticles in spatially confined microfluidic channels.The Review of scientific instruments · 2019Article
- Single-Molecule Flow Platform for the Quantification of Biomolecules Attached to Single Nanoparticles.Analytical chemistry · 2018Article
- New generation of ensemble-decision aliquot ranking based on simplified microfluidic components for large-capacity trapping of circulating tumor cells.Analytical chemistry · 2013Article
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
Recent single-cell and single-molecule studies have shown that a variety of subpopulations exist within biological systems, such as synaptic vesicles, that have previously been overlooked in common bulk studies. By isolating and enriching these various subpopulations, detailed analysis with a variety of analytical techniques can be done to further understand the role that various subpopulations play in cellular dynamics and how alterations to these subpopulations affect the overall function of the biological system. Previous sorters lack the sensitivity, sorting speed, and efficiency to isolate synaptic vesicles and other nanoscale systems. This paper describes the development of a fluorescence-activated nanoscale subcellular sorter that can sort nearly 10 million objects per hour with single-molecule sensitivity. Utilizing a near-nanoscale channel system, we were able to achieve upward of 91% recovery of desired objects with a 99.7% purity.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.