ArticlePloS one2012
Locating the binding sites of Pb(II) ion with human and bovine serum albumins.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 113 citations in OpenAlex.
- Rapid, High-Capacity, and Reusable Bovine Serum Albumin-Based Adsorbents for Perfluoroalkyl and Polyfluoroalkyl Substance Removal.ACS applied materials & interfaces · 2026Article
- Exploring biochemical considerations for diffusive alpha radiation therapy (DaRT) models.Physica medica : PM : an international journal devoted to the applications of physics to medicine and biology : official journal of the Italian Association of Biomedical Physics (AIFB) · 2025Review
- DRGD-linked charged EKKE dimeric dodecapeptide: pH-based amyloid nanostructures and their application in lead and uranium binding.RSC advances · 2024Article
- Simultaneous binding characterization of different chromium speciation to serum albumin.Biometals : an international journal on the role of metal ions in biology, biochemistry, and medicine · 2024Article
- Spectroscopic Analysis of the Effect of Ibuprofen Degradation Products on the Interaction between Ibuprofen and Human Serum Albumin.Current protein & peptide science · 2024Article
- Selective Near-Infrared Blood Detection Driven by Ionic Liquid-Dye-Albumin Nanointeractions.Langmuir : the ACS journal of surfaces and colloids · 2023Article
- Pb(II) coordination to the nonclassical zinc finger tristetraprolin: retained function with an altered fold.Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry · 2023Article
- Effect of Ionic and Non-Ionic Surfactant on Bovine Serum Albumin Encapsulation and Biological Properties of Emulsion-Electrospun Fibers.Molecules (Basel, Switzerland) · 2022Article
- Complete ablation of tumors using synchronous chemoradiation with bimetallic theranostic nanoparticles.Bioactive materials · 2022Article
- Deciphering the Role of π-Interactions in Polyelectrolyte Complexes Using Rationally Designed Peptides.Polymers · 2021Article
- An Overview of Serum Albumin Interactions with Biomedical Alloys.Materials (Basel, Switzerland) · 2020Review
- Bovine Serum Albumin Protein-Based Liquid Crystal Biosensors for Optical Detection of Toxic Heavy Metals in Water.Sensors (Basel, Switzerland) · 2020Article
- Novel Albumin Nanoparticle Enhanced the Anti-Insulin-Resistant-Hepatoma Activity of Metformin.International journal of nanomedicine · 2020Article
- Elucidating the influence of gold nanoparticles on the binding of salvianolic acid B and rosmarinic acid to bovine serum albumin.PloS one · 2015Article
- New insight into molecular interaction of heavy metal pollutant--cadmium(II) with human serum albumin.Environmental science and pollution research international · 2014Article
- Article
- Effect of pullulan nanoparticle surface charges on HSA complexation and drug release behavior of HSA-bound nanoparticles.PloS one · 2012Article
- Probing the binding sites of antibiotic drugs doxorubicin and N-(trifluoroacetyl) doxorubicin with human and bovine serum albumins.PloS one · 2012Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Lead is a potent environmental toxin that has accumulated above its natural level as a result of human activity. Pb cation shows major affinity towards protein complexation and it has been used as modulator of protein-membrane interactions. We located the binding sites of Pb(II) with human serum (HSA) and bovine serum albumins (BSA) at physiological conditions, using constant protein concentration and various Pb contents. FTIR, UV-visible, CD, fluorescence and X-ray photoelectron spectroscopic (XPS) methods were used to analyse Pb binding sites, the binding constant and the effect of metal ion complexation on HSA and BSA stability and conformations. Structural analysis showed that Pb binds strongly to HSA and BSA via hydrophilic contacts with overall binding constants of K(Pb-HSA) = 8.2 (±0.8)×10(4) M(-1) and K(Pb-BSA) = 7.5 (±0.7)×10(4) M(-1). The number of bound Pb cation per protein is 0.7 per HSA and BSA complexes. XPS located the binding sites of Pb cation with protein N and O atoms. Pb complexation alters protein conformation by a major reduction of α-helix from 57% (free HSA) to 48% (metal-complex) and 63% (free BSA) to 52% (metal-complex) inducing a partial protein destabilization.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.