ArticlePloS one2012
NAD-independent L-lactate dehydrogenase is required for L-lactate utilization in Pseudomonas stutzeri SDM.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 34 citations in OpenAlex.
- The L-lactate dehydrogenases ofmBio · 2024Article
- The L-lactate dehydrogenases ofbioRxiv : the preprint server for biology · 2024Article
- Transcriptomics reveals the effect of ammonia nitrogen concentration onSynthetic and systems biotechnology · 2023Article
- Flavin Mononucleotide-Dependent l-Lactate Dehydrogenases: Expanding the Toolbox of Enzymes for l-Lactate Biosensors.ACS omega · 2022Article
- Transcriptional Profiling Reveals Adaptive Response and Tolerance to Lactic Acid Stress inFoods (Basel, Switzerland) · 2022Article
- Production of R- and S-1,2-propanediol in engineered Lactococcus lactis.AMB Express · 2021Article
- Article
- A Bacterial Multidomain NAD-Independent d-Lactate Dehydrogenase Utilizes Flavin Adenine Dinucleotide and Fe-S Clusters as Cofactors and Quinone as an Electron Acceptor for d-Lactate Oxidization.Journal of bacteriology · 2017Article
- Lactate oxidation facilitates growth of Mycobacterium tuberculosis in human macrophages.Scientific reports · 2017Article
- NAD-Independent L-Lactate Dehydrogenase Required for L-Lactate Utilization in Pseudomonas stutzeri A1501.Journal of bacteriology · 2015Article
- Utilization of D-Lactate as an Energy Source Supports the Growth of Gluconobacter oxydans.Applied and environmental microbiology · 2015Article
- A novel biocatalyst for efficient production of 2-oxo-carboxylates using glycerol as the cost-effective carbon source.Biotechnology for biofuels · 2015Article
- Major Role of NAD-Dependent Lactate Dehydrogenases in the Production of l-Lactic Acid with High Optical Purity by the Thermophile Bacillus coagulans.Applied and environmental microbiology · 2014Article
- Reconstruction of lactate utilization system in Pseudomonas putida KT2440: a novel biocatalyst for l-2-hydroxy-carboxylate production.Scientific reports · 2014Article
- Genome Sequence of the Nonpathogenic Pseudomonas aeruginosa Strain ATCC 15442.Genome announcements · 2014Article
- Draft Genome Sequence of the Gluconobacter oxydans Strain DSM 2003, an Important Biocatalyst for Industrial Use.Genome announcements · 2014Article
- New constitutive vectors: useful genetic engineering tools for biocatalysis.Applied and environmental microbiology · 2013Article
- Rationally re-designed mutation of NAD-independent L-lactate dehydrogenase: high optical resolution of racemic mandelic acid by the engineered Escherichia coli.Microbial cell factories · 2012Article
- Genome sequence of the lactate-utilizing Pseudomonas aeruginosa strain XMG.Journal of bacteriology · 2012Article
- Efficient production of pyruvate from DL-lactate by the lactate-utilizing strain Pseudomonas stutzeri SDM.PloS one · 2012Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundVarious Pseudomonas strains can use L-lactate as their sole carbon source for growth. However, the L-lactate-utilizing enzymes in Pseudomonas have never been identified and further studied. METHODOLOGY/PRINCIPAL
findingsAn NAD-independent L-lactate dehydrogenase (L-iLDH) was purified from the membrane fraction of Pseudomonas stutzeri SDM. The enzyme catalyzes the oxidation of L-lactate to pyruvate by using FMN as cofactor. After cloning its encoding gene (lldD), L-iLDH was successfully expressed, purified from a recombinant Escherichia coli strain, and characterized. An lldD mutant of P. stutzeri SDM was constructed by gene knockout technology. This mutant was unable to grow on L-lactate, but retained the ability to grow on pyruvate. CONCLUSIONS/SIGNIFICANCE: It is proposed that L-iLDH plays an indispensable function in Pseudomonas L-lactate utilization by catalyzing the conversion of L-lactate into pyruvate.
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