ArticlePloS one2012
Digital gene expression profiling by 5'-end sequencing of cDNAs during reprogramming in the moss Physcomitrella patens.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 30 citations in OpenAlex.
- STEMIN transcription factor drives selective chromatin remodeling for gene activation within a relaxed chromatin during reprogramming in the moss Physcomitrium patens.The Plant journal : for cell and molecular biology · 2025Article
- The Growing Liberality Observed in Primary Animal and Plant Cultures is Common to the Social Amoeba.Journal of bioinformatics and systems biology : Open access · 2023Article
- Transcriptome Dedifferentiation Observed in Animal Primary Cultures is Essential to Plant Reprogramming.Journal of bioinformatics and systems biology : Open access · 2022Article
- Vacuoles in Bryophytes: Properties, Biogenesis, and Evolution.Frontiers in plant science · 2022Review
- The MossThe Plant cell · 2020Review
- Single-cell transcriptome analysis of Physcomitrella leaf cells during reprogramming using microcapillary manipulation.Nucleic acids research · 2019Article
- Transcriptome Analysis of Male Drosophila melanogaster Exposed to Ethylparaben Using Digital Gene Expression Profiling.Journal of insect science (Online) · 2017Article
- A Lin28 homologue reprograms differentiated cells to stem cells in the moss Physcomitrella patens.Nature communications · 2017Article
- Historical review of research on plant cell dedifferentiation.Journal of plant research · 2015Review
- Specific pools of endogenous peptides are present in gametophore, protonema, and protoplast cells of the moss Physcomitrella patens.BMC plant biology · 2015Article
- Molecular mechanisms of reduced nerve toxicity by titanium dioxide nanoparticles in the phoxim-exposed brain of Bombyx mori.PloS one · 2014Article
- Article
- ppdb: plant promoter database version 3.0.Nucleic acids research · 2014Article
- System for stable β-estradiol-inducible gene expression in the moss Physcomitrella patens.PloS one · 2013Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Stem cells self-renew and repeatedly produce differentiated cells during development and growth. The differentiated cells can be converted into stem cells in some metazoans and land plants with appropriate treatments. After leaves of the moss Physcomitrella patens are excised, leaf cells reenter the cell cycle and commence tip growth, which is characteristic of stem cells called chloronema apical cells. To understand the underlying molecular mechanisms, a digital gene expression profiling method using mRNA 5'-end tags (5'-DGE) was established. The 5'-DGE method produced reproducible data with a dynamic range of four orders that correlated well with qRT-PCR measurements. After the excision of leaves, the expression levels of 11% of the transcripts changed significantly within 6 h. Genes involved in stress responses and proteolysis were induced and those involved in metabolism, including photosynthesis, were reduced. The later processes of reprogramming involved photosynthesis recovery and higher macromolecule biosynthesis, including of RNA and proteins. Auxin and cytokinin signaling pathways, which are activated during stem cell formation via callus in flowering plants, are also activated during reprogramming in P. patens, although no exogenous phytohormone is applied in the moss system, suggesting that an intrinsic phytohormone regulatory system may be used in the moss.
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