ArticlePloS one2012
Association of the chromosome replication initiator DnaA with the Escherichia coli inner membrane in vivo: quantity and mode of binding.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed.
- A novel membrane stress response that blocks chromosomal replication by targeting the DnaA initiator via the ClpP protease.Journal of bacteriology · 2025Article
- Review
- Review
- Relative Distribution of DnaA and DNA inInternational journal of molecular sciences · 2025Article
- Hypothesis: bacteria live on the edge of phase transitions with a cell cycle regulated by a water-clock.Theory in biosciences = Theorie in den Biowissenschaften · 2024Article
- Open Questions about the Roles of DnaA, Related Proteins, and Hyperstructure Dynamics in the Cell Cycle.Life (Basel, Switzerland) · 2023Review
- Recollections of a Helmstetter Disciple.Life (Basel, Switzerland) · 2023Review
- The linker domain of the initiator DnaA contributes to its ATP binding and membrane association inScience advances · 2022Article
- Proteome based mapping and molecular docking revealed DnaA as a potential drug target againstSaudi journal of biological sciences · 2022Article
- Link Between Antibiotic Persistence and Antibiotic Resistance in Bacterial Pathogens.Frontiers in cellular and infection microbiology · 2022Review
- Stochastic nucleoid segregation dynamics as a source of the phenotypic variability in E. coli.Biophysical journal · 2021Article
- Centrifugal sedimentation immunoassays for multiplexed detection of enteric bacteria in ground water.Biomicrofluidics · 2016Article
- Skin-Derived C-Terminal Filaggrin-2 Fragments Are Pseudomonas aeruginosa-Directed Antimicrobials Targeting Bacterial Replication.PLoS pathogens · 2015Article
- N-terminal-mediated oligomerization of DnaA drives the occupancy-dependent rejuvenation of the protein on the membrane.Bioscience reports · 2015Article
- The orisome: structure and function.Frontiers in microbiology · 2015Review
- The membrane: transertion as an organizing principle in membrane heterogeneity.Frontiers in microbiology · 2015Review
- Crosstalk between DnaA protein, the initiator of Escherichia coli chromosomal replication, and acidic phospholipids present in bacterial membranes.International journal of molecular sciences · 2013Review
- Chromosome Replication in Escherichia coli: Life on the Scales.Life (Basel, Switzerland) · 2012Article
- Chromosomal replication initiation machinery of low-G+C-content Firmicutes.Journal of bacteriology · 2012Review
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
DnaA initiates chromosome replication in most known bacteria and its activity is controlled so that this event occurs only once every cell division cycle. ATP in the active ATP-DnaA is hydrolyzed after initiation and the resulting ADP is replaced with ATP on the verge of the next initiation. Two putative recycling mechanisms depend on the binding of DnaA either to the membrane or to specific chromosomal sites, promoting nucleotide dissociation. While there is no doubt that DnaA interacts with artificial membranes in vitro, it is still controversial as to whether it binds the cytoplasmic membrane in vivo. In this work we looked for DnaA-membrane interaction in E. coli cells by employing cell fractionation with both native and fluorescent DnaA hybrids. We show that about 10% of cellular DnaA is reproducibly membrane-associated. This small fraction might be physiologically significant and represent the free DnaA available for initiation, rather than the vast majority bound to the datA reservoir. Using the combination of mCherry with a variety of DnaA fragments, we demonstrate that the membrane binding function is delocalized on the surface of the protein's domain III, rather than confined to a particular sequence. We propose a new binding-bending mechanism to explain the membrane-induced nucleotide release from DnaA. This mechanism would be fundamental to the initiation of replication.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.