ArticlePloS one2012
Quantification of unintegrated HIV-1 DNA at the single cell level in vivo.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
19 citing papers in PubMed.
- Single cell analysis of lymph node tissue from HIV-1 infected patients reveals that the majority of CD4+ T-cells contain one HIV-1 DNA molecule.PLoS pathogens · 2013Trial
- Expression of HIV from a 1-LTR circular DNA in the absence of integration.Retrovirology · 2025Article
- Epistatic pathways can drive HIV-1 escape from integrase strand transfer inhibitors.Science advances · 2024Article
- Quantification of Total HIV DNA as a Marker to Measure Viral Reservoir: Methods and Potential Implications for Clinical Practice.Diagnostics (Basel, Switzerland) · 2021Review
- Early ART-initiation and longer ART duration reduces HIV-1 proviral DNA levels in children from the CHER trial.AIDS research and therapy · 2021Article
- Article
- Gut and blood differ in constitutive blocks to HIV transcription, suggesting tissue-specific differences in the mechanisms that govern HIV latency.PLoS pathogens · 2018Article
- Review
- Reduced antiretroviral drug efficacy and concentration in HIV-infected microglia contributes to viral persistence in brain.Retrovirology · 2017Article
- Modeling brain lentiviral infections during antiretroviral therapy in AIDS.Journal of neurovirology · 2017Article
- Underlying mechanisms of HIV-1 latency.Virus genes · 2017Review
- The Alphabet Soup of HIV Reservoir Markers.Current HIV/AIDS reports · 2017Review
- Impact of Different Antiretroviral Strategies on Total HIV-DNA Level in Virologically Suppressed HIV-1 Infected Patients.Current HIV research · 2017Article
- Single-Cell Genomics for Virology.Viruses · 2016Review
- Subversion of Cell Cycle Regulatory Mechanisms by HIV.Cell host & microbe · 2015Review
- Thymic HIV-2 infection uncovers posttranscriptional control of viral replication in human thymocytes.Journal of virology · 2015Article
- Article
- Determining the cellular diversity of hepatitis C virus quasispecies by single-cell viral sequencing.Journal of virology · 2013Article
- An HIV-1 replication pathway utilizing reverse transcription products that fail to integrate.Journal of virology · 2013Article
Corrections and comments
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Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In the nucleus of HIV-1 infected cells, unintegrated HIV-1 DNA molecules exist in the form of one and two LTR circles and linear molecules with degraded extremities. In tissue culture they are invariably more numerous than the provirus, the relative proportion of integrated to unintegrated forms varies widely from ∼1:1 to 1:10 and even over 1:100. In vivo, this ratio is unknown. To determine it, single nuclei from two infected patients with a known provirus copy number were microdissected, HIV DNA was amplified by nested PCR, cloned and individual clones sequenced. Given the extraordinary sequence complexity, we made the assumption that the total number of distinct sequences approximated to real number of amplifiable HIV-1 DNA templates in the nucleus. We found that the number of unintegrated DNA molecules increased linearly with the proviral copy number there being on average 86 unintegrated molecules per provirus.
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Registered trials
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