ArticleSensors (Basel, Switzerland)2009
EROD and MROD as Markers of Cytochrome P450 1A Activities in Hepatic Microsomes from Entire and Castrated Male Pigs.
Article in Sensors (Basel, Switzerland), 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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15 citing papers in PubMed, 64 citations in OpenAlex.
- Biocompatibility of the oxygen carrier polymerized human hemoglobin towards HepG2/C3A cells.Heliyon · 2023Article
- Impact of valproic acid on busulfan pharmacokinetics: In vitro assessment of potential drug-drug interaction.PloS one · 2023Article
- Metabolism andInternational journal of occupational medicine and environmental health · 2022Article
- Stability investigations of cytochrome P450 (CYP) enzymes immediately after death in a pig model support the applicability of postmortem hepatic CYP quantification.Pharmacology research & perspectives · 2021Article
- Novel Synthetic Analogues of 19(S/R)-Hydroxyeicosatetraenoic Acid Exhibit Noncompetitive Inhibitory Effect on the Activity of Cytochrome P450 1A1 and 1B1.European journal of drug metabolism and pharmacokinetics · 2021Article
- Induction of Cyp450 enzymes by 4-thiazolidinone-based derivatives in 3T3-L1 cells in vitro.Naunyn-Schmiedeberg's archives of pharmacology · 2021Article
- Neurovascular Drug Biotransformation Machinery in Focal Human Epilepsies: Brain CYP3A4 Correlates with Seizure Frequency and Antiepileptic Drug Therapy.Molecular neurobiology · 2019Article
- Effect of taurine on chronic and acute liver injury: Focus on blood and brain ammonia.Toxicology reports · 2016Article
- In vitro gender-dependent inhibition of porcine cytochrome p450 activity by selected flavonoids and phenolic acids.BioMed research international · 2015Article
- Regulation of porcine hepatic cytochrome p450 - implication for boar taint.Computational and structural biotechnology journal · 2014Review
- In vitro inhibition of 7-ethoxyresorufin-O-deethylase (EROD) and p-nitrophenol hydroxylase (PNPH) activities by sesamin in hepatic microsomes from two fish species.Molecular biology reports · 2013Article
- Simultaneous determination of cytochrome P450 1A, 2A and 3A activities in porcine liver microsomes.Interdisciplinary toxicology · 2012Article
- Tolbutamide hydroxylation by hepatic microsomes from Atlantic salmon (Salmo salar L.).Molecular biology reports · 2012Article
- Para-nitrophenol hydroxylation by fish liver microsomes: kinetics and effect of selective cytochrome P450 inhibitors.Fish physiology and biochemistry · 2011Article
- In vitro inhibition of porcine cytochrome P450 by 17β -estradiol and 17α-estradiol.Interdisciplinary toxicology · 2011Article
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2 authors at 1 institution in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
In the present study, we characterized the kinetic parameters of 7-ethoxy-resorufin O-deethylation (EROD) and 7-methoxyresorufin O-demethylation (MROD) in hepatic microsomes from entire and castrated male pigs. Validation parameters of an HPLC-based method to analyse EROD and MROD activities are also described. Eadie-Hofstee plot analysis demonstrated a biphasic kinetic of EROD, indicating that at least two forms of cytochrome P450 are involved in this reaction. MROD followed monophasic kinetic, suggesting that a single enzyme, or enzymes with similar affinities, is responsible for the reaction. Inhibitory effects of α-naphthoflavone (ANF), ellipticine and furafylline were studied using microsomes from entire and castrated male pigs. ANF is a known inhibitor of both cytochrome P(450) 1A1 and 1A2 (CYP1A1 and CYP1A2); the presence of ANF in the incubations resulted in the inhibition of both EROD and MROD activities in porcine liver microsomes. EROD activities in porcine liver microsomes were also inhibited by selective CYP1A1 inhibitor ellipticine, but not by CYP1A2 inhibitor furafylline. MROD activities were strongly inhibited by ellipticine and to a much lesser extent by furafylline. Further studies are needed to evaluate substrate specificities of porcine CYP1A1 and CYP1A2.
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