ArticleMolecular biology of the cell2012
A role for myosin IXb, a motor-RhoGAP chimera, in epithelial wound healing and tight junction regulation.
Article in Molecular biology of the cell, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
32 citing papers in PubMed, 46 citations in OpenAlex.
- Myosin-9b Controls Epithelial Brush Border Architecture through Motility-Dependent RhoA Signaling.bioRxiv : the preprint server for biology · 2026Article
- Review
- Single cell and spatial characterization of the human pancreas reveals drivers of beta cell dysfunction in cystic fibrosis.bioRxiv : the preprint server for biology · 2025Article
- Exploring the shared genetic basis between autism spectrum disorder and gastrointestinal disorders: a bioinformatic study.Scientific reports · 2025Article
- Myosin IXB protects immune cells from virus infection.The Journal of general virology · 2025Article
- An allosteric inhibitor of RhoGAP class-IX myosins suppresses the metastatic features of cancer cells.Nature communications · 2024Article
- Role of Rho GTPases in inflammatory bowel disease.Cell death discovery · 2023Review
- The myosin and RhoGAP MYO9B influences osteocyte dendrite growth and responses to mechanical stimuli.Frontiers in bioengineering and biotechnology · 2023Article
- Impact of Epithelial Cell Shedding on Intestinal Homeostasis.International journal of molecular sciences · 2022Review
- Effect of Monoacylglycerol Lipase Inhibition on Intestinal Permeability of Rats With Severe Acute Pancreatitis.Frontiers in pharmacology · 2022Article
- The Correlation Between MYO9B Gene Polymorphism and Inflammatory Bowel Disease in the Guangxi Zhuang Population.International journal of general medicine · 2021Article
- SLIT2/ROBO1-signaling inhibits macropinocytosis by opposing cortical cytoskeletal remodeling.Nature communications · 2020Article
- The RhoGAP HUM-7/Myo9 integrates signals to modulate RHO-1/RhoA during embryonic morphogenesis inDevelopment (Cambridge, England) · 2018Article
- Myosins in Osteoclast Formation and Function.Biomolecules · 2018Review
- Cell-Cell Junctions Organize Structural and Signaling Networks.Cold Spring Harbor perspectives in biology · 2018Review
- Molecular pathways driving disease-specific alterations of intestinal epithelial cells.Cellular and molecular life sciences : CMLS · 2017Review
- Celiac Disease: Role of the Epithelial Barrier.Cellular and molecular gastroenterology and hepatology · 2017Review
- The intestinal epithelial barrier: a therapeutic target?Nature reviews. Gastroenterology & hepatology · 2017Review
- Interplay of GTPases and Cytoskeleton in Cellular Barrier Defects during Gut Inflammation.Frontiers in immunology · 2017Review
- Arhgap17, a RhoGTPase activating protein, regulates mucosal and epithelial barrier function in the mouse colon.Scientific reports · 2016Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Polymorphisms in the gene encoding the heavy chain of myosin IXb (Myo9b) have been linked to several forms of inflammatory bowel disease (IBD). Given that Myo9b contains a RhoGTPase-activating protein domain within its tail, it may play key roles in Rho-mediated actin cytoskeletal modifications critical to intestinal barrier function. In wounded monolayers of the intestinal epithelial cell line Caco2(BBe) (BBe), Myo9b localizes to the extreme leading edge of lamellipodia of migrating cells. BBe cells exhibiting loss of Myo9b expression with RNA interference or Myo9b C-terminal dominant-negative (DN) tail-tip expression lack lamellipodia, fail to migrate into the wound, and form stress fiber-like arrays of actin at the free edges of cells facing the wound. These cells also exhibit disruption of tight junction (TJ) protein localization, including ZO-1, occludin, and claudin-1. Torsional motility and junctional permeability to dextran are greatly increased in cells expressing DN-tail-tip. Of interest, this effect is propagated to neighboring cells. Consistent with a role for Myo9b in regulating levels of active Rho, localization of both RhoGTP and myosin light chain phosphorylation corresponds to Myo9b-knockdown regions of BBe monolayers. These data reveal critical roles for Myo9b during epithelial wound healing and maintenance of TJ integrity-key functions that may be altered in patients with Myo9b-linked IBD.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.