ArticleJournal of virology2012
Crystal structure of the superfamily 1 helicase from Tomato mosaic virus.
Article in Journal of virology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 57 citations in OpenAlex.
- Structure, Function and Inhibition of Helicases Involved in Virus Infection.Biomolecules · 2026Review
- Detection of bacterial gene expression elements on Tobacco mosaic virus RNA using cDNA analysis.PloS one · 2026Article
- Tobacco Mosaic Virus Movement: From Capsid Disassembly to Transport Through Plasmodesmata.Viruses · 2025Review
- Methotrexate, an anti-inflammatory drug, inhibits Hepatitis E viral replication.Journal of enzyme inhibition and medicinal chemistry · 2023Article
- Uncovering a Complex Virome Associated with the Cacao PathogensPathogens (Basel, Switzerland) · 2023Article
- The impact of the suppression of highly connected protein interactions on the corona virus infection.Scientific reports · 2022Article
- Review
- Insights into SARS-CoV-2 genome, structure, evolution, pathogenesis and therapies: Structural genomics approach.Biochimica et biophysica acta. Molecular basis of disease · 2020Review
- Review on Structures of Pesticide Targets.International journal of molecular sciences · 2020Review
- Subdomain cryo-EM structure of nodaviral replication protein A crown complex provides mechanistic insights into RNA genome replication.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Structural Characterization of the Helicase nsp10 Encoded by Porcine Reproductive and Respiratory Syndrome Virus.Journal of virology · 2020Article
- Structural insights into RNA recognition by the Chikungunya virus nsP2 helicase.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Binding studies between cytosinpeptidemycin and the superfamily 1 helicase protein of tobacco mosaic virus.RSC advances · 2018Article
- Molecular Analysis and Modeling of Hepatitis E Virus Helicase and Identification of Novel Inhibitors by Virtual Screening.BioMed research international · 2018Article
- Organelle luminal dependence of (+)strand RNA virus replication reveals a hidden druggable target.Science advances · 2018Article
- Viral Macro Domains Reverse Protein ADP-Ribosylation.Journal of virology · 2016Article
- What we know but do not understand about nidovirus helicases.Virus research · 2015Review
- Structural basis for the recognition-evasion arms race between Tomato mosaic virus and the resistance gene Tm-1.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Functional characterization of the mutations in Pepper mild mottle virus overcoming tomato tm-1-mediated resistance.Molecular plant pathology · 2014Article
- Structural basis for the regulatory function of a complex zinc-binding domain in a replicative arterivirus helicase resembling a nonsense-mediated mRNA decay helicase.Nucleic acids research · 2014Article
Corrections and comments
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Authors and funding
9 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The genomes of the Tomato mosaic virus and many other plant and animal positive-strand RNA viruses of agronomic and medical importance encode superfamily 1 helicases. Although helicases play important roles in viral replication, the crystal structures of viral superfamily 1 helicases have not been determined. Here, we report the crystal structure of a fragment (S666 to Q1116) of the replication protein from Tomato mosaic virus. The structure reveals a novel N-terminal domain tightly associated with a helicase core. The helicase core contains two RecA-like α/β domains without any of the accessory domain insertions that are found in other superfamily 1 helicases. The N-terminal domain contains a flexible loop, a long α-helix, and an antiparallel six-stranded β-sheet. On the basis of the structure, we constructed deletion mutants of the S666-to-Q1116 fragment and performed split-ubiquitin-based interaction assays in Saccharomyces cerevisiae with TOM1 and ARL8, host proteins that are essential for tomato mosaic virus RNA replication. The results suggested that both TOM1 and ARL8 interact with the long α-helix in the N-terminal domain and that TOM1 also interacts with the helicase core. Prediction of secondary structures in other viral superfamily 1 helicases and comparison of those structures with the S666-to-Q1116 structure suggested that these helicases have a similar fold. Our results provide a structural basis of viral superfamily 1 helicases.
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