ArticleJournal of cell science2012
A FAP46 mutant provides new insights into the function and assembly of the C1d complex of the ciliary central apparatus.
Article in Journal of cell science, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
32 citing papers in PubMed, 52 citations in OpenAlex.
- Heterogeneity of radial spoke components in Tetrahymena cilia.Cellular and molecular life sciences : CMLS · 2025Article
- Cilia and transcription: a mini review.Frontiers in cell and developmental biology · 2025Review
- Pathogenic variants inThe European respiratory journal · 2024Article
- Structural and molecular distinctions of primary and secondary spines in the sea urchin Lytechinus variegatus.Scientific reports · 2024Article
- Lack of CFAP54 causes primary ciliary dyskinesia in a mouse model and human patients.Frontiers of medicine · 2023Article
- Concurrent loss of ciliary genesRoyal Society open science · 2023Article
- Cryo-EM structure of an active central apparatus.Nature structural & molecular biology · 2022Article
- Ciliary central apparatus structure reveals mechanisms of microtubule patterning.Nature structural & molecular biology · 2022Article
- Ependymal Cilia: Physiology and Role in Hydrocephalus.Frontiers in molecular neuroscience · 2022Review
- Structural organization of the C1b projection within the ciliary central apparatus.Journal of cell science · 2021Article
- Chlamydomonas FAP70 is a component of the previously uncharacterized ciliary central apparatus projection C2a.Journal of cell science · 2021Article
- Composition and function of the C1b/C1f region in the ciliary central apparatus.Scientific reports · 2021Article
- Autosomal recessive loci contribute significantly to quantitative variation of male fertility in a dairy cattle population.BMC genomics · 2021Article
- Central Apparatus, the Molecular Kickstarter of Ciliary and Flagellar Nanomachines.International journal of molecular sciences · 2021Review
- Analysis of the sperm flagellar axoneme using gene-modified mice.Experimental animals · 2020Review
- TIM, a targeted insertional mutagenesis method utilizing CRISPR/Cas9 in Chlamydomonas reinhardtii.PloS one · 2020Article
- Identification and mapping of central pair proteins by proteomic analysis.Biophysics and physicobiology · 2020Article
- Rare Human Diseases: Model Organisms in Deciphering the Molecular Basis of Primary Ciliary Dyskinesia.Cells · 2019Review
- Structural organization of the C1a-e-c supercomplex within the ciliary central apparatus.The Journal of cell biology · 2019Article
- Review
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
Virtually all motile eukaryotic cilia and flagella have a '9+2' axoneme in which nine doublet microtubules surround two singlet microtubules. Associated with the central pair of microtubules are protein complexes that form at least seven biochemically and structurally distinct central pair projections. Analysis of mutants lacking specific projections has indicated that each may play a unique role in the control of flagellar motility. One of these is the C1d projection previously shown to contain the proteins FAP54, FAP46, FAP74 and FAP221/Pcdp1, which exhibits Ca(2+)-sensitive calmodulin binding. Here we report the isolation and characterization of a Chlamydomonas reinhardtii null mutant for FAP46. This mutant, fap46-1, lacks the C1d projection and has impaired motility, confirming the importance of this projection for normal flagellar movement. Those cells that are motile have severe defects in phototaxis and the photoshock response, underscoring a role for the C1d projection in Ca(2+)-mediated flagellar behavior. The data also reveal for the first time that the C1d projection is involved in the control of interdoublet sliding velocity. Our studies further identify a novel C1d subunit that we term C1d-87, give new insight into relationships between the C1d subunits, and provide evidence for multiple sites of calmodulin interaction within the C1d projection. These results represent significant advances in our understanding of an important but little studied axonemal structure.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.