ArticleProceedings of the National Academy of Sciences of the United States of America2012
Active DNA unwinding dynamics during processive DNA replication.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 51 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
51 citing papers in PubMed.
- Improved Sensitivity of Quantitative Polymerase Chain Reaction and Next Generation Sequencing for Detection of Salmonella spp. in Mixed Environmental Communities Using Whole Genome Amplification.MicrobiologyOpen · 2025Article
- Autoregulation of the real-time kinetics of the human mitochondrial replicative helicase.Nature communications · 2025Article
- Direct visualization of replication and R-loop collision using single-molecule imaging.Nucleic acids research · 2024Article
- Genome replication in asynchronously growing microbial populations.PLoS computational biology · 2024Article
- Revealing an initiation inhibition of RCA and its application in nucleic acid detection.Acta biochimica et biophysica Sinica · 2023Article
- Mechanism of strand displacement DNA synthesis by the coordinated activities of human mitochondrial DNA polymerase and SSB.Nucleic acids research · 2023Article
- DNA Polymerase-Parental DNA Interaction Is Essential for Helicase-Polymerase Coupling during Bacteriophage T7 DNA Replication.International journal of molecular sciences · 2022Article
- Conformational Dynamics of DNA Polymerases Revealed at the Single-Molecule Level.Frontiers in molecular biosciences · 2022Review
- Formation and Repair of an Interstrand DNA Cross-Link Arising from a Common Endogenous Lesion.Journal of the American Chemical Society · 2021Article
- The nucleotide addition cycle of the SARS-CoV-2 polymerase.Cell reports · 2021Article
- Review
- The nucleotide addition cycle of the SARS-CoV-2 polymerase.bioRxiv : the preprint server for biology · 2021Article
- The Single-Cell Sequencing: A Dazzling Light Shining on the Dark Corner of Cancer.Frontiers in oncology · 2021Review
- DNA replication:Computational and structural biotechnology journal · 2021Review
- DNA replication machinery: Insights fromComputational and structural biotechnology journal · 2021Review
- Dynamics of Staphylococcus aureus Cas9 in DNA target Association and Dissociation.EMBO reports · 2020Article
- Review
- Replicative DNA polymerases promote active displacement of SSB proteins during lagging strand synthesis.Nucleic acids research · 2019Article
- The mechanism of DNA unwinding by the eukaryotic replicative helicase.Nature communications · 2019Article
- New insights into the coordination between the polymerization and 3'-5' exonuclease activities in ϕ29 DNA polymerase.Scientific reports · 2019Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Duplication of double-stranded DNA (dsDNA) requires a fine-tuned coordination between the DNA replication and unwinding reactions. Using optical tweezers, we probed the coupling dynamics between these two activities when they are simultaneously carried out by individual Phi29 DNA polymerase molecules replicating a dsDNA hairpin. We used the wild-type and an unwinding deficient polymerase variant and found that mechanical tension applied on the DNA and the DNA sequence modulate in different ways the replication, unwinding rates, and pause kinetics of each polymerase. However, incorporation of pause kinetics in a model to quantify the unwinding reaction reveals that both polymerases destabilize the fork with the same active mechanism and offers insights into the topological strategies that could be used by the Phi29 DNA polymerase and other DNA replication systems to couple unwinding and replication reactions.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.