ArticleCytoskeleton (Hoboken, N.J.)2012
Computer-assisted image analysis of human cilia and Chlamydomonas flagella reveals both similarities and differences in axoneme structure.
Article in Cytoskeleton (Hoboken, N.J.), 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
20 citing papers in PubMed, 39 citations in OpenAlex.
- Systematic review of artificial intelligence and digital-automated tools for screening, diagnosis and characterisation of primary ciliary dyskinesia.ERJ open research · 2026Article
- The ERK activator, BCI, inhibits ciliogenesis and causes defects in motor behavior, ciliary gating, and cytoskeletal rearrangement.Life science alliance · 2023Article
- Motile cilia genetics and cell biology: big results from little mice.Cellular and molecular life sciences : CMLS · 2021Review
- Distinct architecture and composition of mouse axonemal radial spoke head revealed by cryo-EM.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- CFAP45 deficiency causes situs abnormalities and asthenospermia by disrupting an axonemal adenine nucleotide homeostasis module.Nature communications · 2020Article
- A novelJournal of genetics · 2020Article
- FAP57/WDR65 targets assembly of a subset of inner arm dyneins and connects to regulatory hubs in cilia.Molecular biology of the cell · 2019Article
- Ciliary Motility: Regulation of Axonemal Dynein Motors.Cold Spring Harbor perspectives in biology · 2017Review
- Cilia and Mucociliary Clearance.Cold Spring Harbor perspectives in biology · 2017Review
- Evolution of Cilia.Cold Spring Harbor perspectives in biology · 2017Review
- Alcohol-induced ciliary dysfunction targets the outer dynein arm.American journal of physiology. Lung cellular and molecular physiology · 2015Article
- Recent advances in primary ciliary dyskinesia genetics.Journal of medical genetics · 2015Review
- Cryo-electron tomography reveals ciliary defects underlying human RSPH1 primary ciliary dyskinesia.Nature communications · 2014Article
- Article
- Combined exome and whole-genome sequencing identifies mutations in ARMC4 as a cause of primary ciliary dyskinesia with defects in the outer dynein arm.Journal of medical genetics · 2014Article
- Article
- Sperm-associated antigen-17 gene is essential for motile cilia function and neonatal survival.American journal of respiratory cell and molecular biology · 2013Article
- Mutations in CCDC39 and CCDC40 are the major cause of primary ciliary dyskinesia with axonemal disorganization and absent inner dynein arms.Human mutation · 2013Article
- The nexin-dynein regulatory complex subunit DRC1 is essential for motile cilia function in algae and humans.Nature genetics · 2013Article
- Conserved structural motifs in the central pair complex of eukaryotic flagella.Cytoskeleton (Hoboken, N.J.) · 2013Article
Corrections and comments
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Authors and funding
4 authors at 3 institutions in 1 country.
Funding
Abstract
In the past decade, investigations from several different fields have revealed the critical role of cilia in human health and disease. Because of the highly conserved nature of the basic axonemal structure, many different model systems have proven useful for the study of ciliopathies, especially the unicellular, biflagellate green alga Chlamydomonas reinhardtii. Although the basic axonemal structure of cilia and flagella is highly conserved, these organelles often perform specialized functions unique to the cell or tissue in which they are found. These differences in function are likely reflected in differences in structural organization. In this work, we directly compare the structure of isolated axonemes from human cilia and Chlamydomonas flagella to identify similarities and differences that potentially play key roles in determining their functionality. Using transmission electron microscopy and 2D image averaging techniques, our analysis has confirmed the overall structural similarity between these two species, but also revealed clear differences in the structure of the outer dynein arms, the central pair projections, and the radial spokes. We also show how the application of 2D image averaging can clarify the underlying structural defects associated with primary ciliary dyskinesia (PCD). Overall, our results document the remarkable similarity between these two structures separated evolutionarily by over a billion years, while highlighting several significant differences, and demonstrate the potential of 2D image averaging to improve the diagnosis and understanding of PCD.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.