ArticleJournal of clinical microbiology2012
Identification of bacterial pathogens in ascitic fluids from patients with suspected spontaneous bacterial peritonitis by use of broad-range PCR (16S PCR) coupled with high-resolution melt analysis.
Article in Journal of clinical microbiology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
22 citing papers in PubMed, 49 citations in OpenAlex.
- Clinical evaluation of multiplex pathogen real-time PCR for early detection of pathogens and antimicrobial resistance genes in intra-abdominal infections.World journal of microbiology & biotechnology · 2026Article
- Diagnostic performance of broad-range PCR in bacterial peritonitis.Frontiers in cellular and infection microbiology · 2025Article
- 16S rRNA Next-Generation Sequencing May Not Be Useful for Examining Suspected Cases of Spontaneous Bacterial Peritonitis.Medicina (Kaunas, Lithuania) · 2024Article
- Diagnosis of Bloodstream Infections: An Evolution of Technologies towards Accurate and Rapid Identification and Antibiotic Susceptibility Testing.Antibiotics (Basel, Switzerland) · 2022Review
- Efficacy of 16S rRNA variable regions high-resolution melt analysis for bacterial pathogens identification in periprosthetic joint infections.BMC microbiology · 2021Article
- Rapid and sensitive identification of pleural and peritoneal infections by droplet digital PCR.Folia microbiologica · 2021Article
- Bacterial Pathogens, Drug-Resistance Profile and Its Associated Factors from Patients with Suspected Peritonitis in Southern Ethiopia.Infection and drug resistance · 2021Article
- Microbial Infections as a Trigger for Acute-on-Chronic Liver Failure: A Review.Medical science monitor : international medical journal of experimental and clinical research · 2019Review
- Emerging Analytical Techniques for Rapid Pathogen Identification and Susceptibility Testing.Annual review of analytical chemistry (Palo Alto, Calif.) · 2019Review
- Combination of vial culture and broad-range PCR for the diagnosis of spontaneous bacterial peritonitis: experience in a Greek tertiary care hospital.New microbes and new infections · 2019Article
- Spontaneous pneumococcal peritonitis diagnosed by qPCR.IDCases · 2019Article
- A "Culture" Shift: Broad Bacterial Detection, Identification, and Antimicrobial Susceptibility Testing Directly from Whole Blood.Clinical chemistry · 2018Article
- Amplification of bacterial genomic DNA from all ascitic fluids with a highly sensitive polymerase chain reaction.Molecular medicine reports · 2018Article
- Massively parallel digital high resolution melt for rapid and absolutely quantitative sequence profiling.Scientific reports · 2017Article
- Microbial Typing by Machine Learned DNA Melt Signatures.Scientific reports · 2017Article
- Microwave-accelerated method for ultra-rapid extraction of Neisseria gonorrhoeae DNA for downstream detection.Analytical biochemistry · 2016Article
- Nested Machine Learning Facilitates Increased Sequence Content for Large-Scale Automated High Resolution Melt Genotyping.Scientific reports · 2016Article
- Application of qualitative and quantitative real-time PCR, direct sequencing, and terminal restriction fragment length polymorphism analysis for detection and identification of polymicrobial 16S rRNA genes in ascites.Journal of clinical microbiology · 2014Article
- Sensitive detection and serovar differentiation of typhoidal and nontyphoidal Salmonella enterica species using 16S rRNA Gene PCR coupled with high-resolution melt analysis.The Journal of molecular diagnostics : JMD · 2014Article
- [Characterization of bacterial flora in community peritonitis carried out in Burkina Faso].The Pan African medical journal · 2014Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 4 institutions in 2 countries.
Funding
Abstract
Spontaneous bacterial peritonitis (SBP) can be a severe complication occurring in patients with cirrhosis and ascites, with associated mortality often as high as 40%. Traditional diagnostics for SBP rely on culture techniques for proper diagnosis, although recent reports suggest that the presence of bacterial DNA in peritoneal fluid in patients with cirrhosis and ascites is an indicator of SBP. A previously published broad-range PCR (16S PCR) coupled with high-resolution melt analysis (HRMA) was compared with standard culture techniques for diagnosis of SBP in 106 peritoneal fluid samples from patients with suspected SBP. The sensitivity and specificity for 16S PCR for detecting eubacterial DNA compared with those of standard culture techniques were 100% (17/17) and 91.5% (85/89), respectively. Overall, HRMA concordance with species identification was 70.6% (12/17), although the 5 samples that were discordant at the species level were SBP resulting from a polymicrobial infection, and species-level identification for polymicrobial infections is outside the capability of HRMA. Both the broad-range 16S PCR and HRMA analysis provide useful diagnostic adjunctive assays for clinicians in detecting and identifying pathogens responsible for SBP.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.