ArticleDigestive diseases and sciences2012
Adenine induces differentiation of rat hepatic stellate cells.
Article in Digestive diseases and sciences, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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Who cites it
7 citing papers in PubMed, 13 citations in OpenAlex.
- Mechanisms of the action of adenine on anti-allergic effects in mast cells.Immunity, inflammation and disease · 2018Article
- Adenine attenuates the Ca(2+) contraction-signaling pathway via adenine receptor-mediated signaling in rat vascular smooth muscle cells.Naunyn-Schmiedeberg's archives of pharmacology · 2016Article
- Cellular localization of adenine receptors in the rat kidney and their functional significance in the inner medullary collecting duct.American journal of physiology. Renal physiology · 2013Article
- The rat adenine receptor: pharmacological characterization and mutagenesis studies to investigate its putative ligand binding site.Purinergic signalling · 2013Article
- Characterization of new G protein-coupled adenine receptors in mouse and hamster.Purinergic signalling · 2013Article
- Translating an understanding of the pathogenesis of hepatic fibrosis to novel therapies.Clinical gastroenterology and hepatology : the official clinical practice journal of the American Gastroenterological Association · 2013Review
- A new method to isolate and culture rat kupffer cells.PloS one · 2013Article
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Authors and funding
5 authors at 3 institutions in 2 countries.
Funding
Abstract
BACKGROUND AND
aimsAdenine is a uric acid pathway metabolite of no known function, and has recently been identified as a ligand for a rat G protein-coupled receptor. Due to the known role of other uric acid pathway metabolites in HSC biology, we tested the ability of adenine to induce HSC differentiation.
methodsRT-PCR was performed for adenine receptor expression in T-6 and primary rat HSC. T-6 and primary rats HSC were cultured with and without adenine, and stellation examined. Next, we examined inhibition of calcium signaling using caged IP(3). To test if adenine inhibits HSC chemotaxis T-6 cells and rat HSCs were cultured with or without adenine for 24 h in a transwell assay with PDGF as the chemoattractant. cDNA was prepared from T-6 and primary HSC for quantification of collagen 1 mRNA using real-time PCR.
resultsWe found that mRNA for the adenine receptor is expressed in T-6 cells and primary rat HSC. Also, adenine induces HSC stellation and adenine inhibits IP(3) mediated increase in cytosolic [Ca(2+)](i) and inhibits chemotaxis in T-6 cells and primary rat HSC. Adenine was also shown to up-regulate α-SMA and collagen 1, and this effect is lost by using specific si-RNA for the adenine receptor. Finally, adenine inhibits endothelin-1-induced gel contraction.
conclusionsThe adenine receptor is present in T-6 cells and primary rats HSC. Adenine, via the adenine receptor, induces morphological change, and cytosolic calcium signaling, inhibits chemotaxis, and up-regulates collagen 1 mRNA in HSCs.
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