ArticleMolecular microbiology2012
Francisella tularensis tmRNA system mutants are vulnerable to stress, avirulent in mice, and provide effective immune protection.
Article in Molecular microbiology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
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Who cites it
22 citing papers in PubMed, 43 citations in OpenAlex.
- The regulation of AsfR on tmRNA expression mediates bacterial motility and virulence inVirulence · 2026Article
- IscR-tmRNA regulatory axis plays a key role in multiple stress response and pathogenicity inVirulence · 2025Article
- Identification and initial characterization of Hfq-associated sRNAs in Histophilus somni strain 2336.PloS one · 2023Article
- Prophage excision switches the primary ribosome rescue pathway and rescue-associated gene regulations in Escherichia coli.Molecular microbiology · 2023Article
- Ribosome Rescue Pathways in Bacteria.Frontiers in microbiology · 2021Review
- Dual RNA-seq of Orientia tsutsugamushi informs on host-pathogen interactions for this neglected intracellular human pathogen.Nature communications · 2020Article
- Article
- Anti-tubercular Activity of Pyrazinamide is Independent of trans-Translation and RpsA.Scientific reports · 2017Article
- Targeting Inhibition of SmpB by Peptide Aptamer Attenuates the Virulence to Protect Zebrafish againstFrontiers in microbiology · 2017Article
- Inhibitors of Ribosome Rescue Arrest Growth of Francisella tularensis at All Stages of Intracellular Replication.Antimicrobial agents and chemotherapy · 2016Article
- Human Cells Require Non-stop Ribosome Rescue Activity in Mitochondria.PLoS genetics · 2016Article
- Absence of tmRNA Has a Protective Effect against Fluoroquinolones inFrontiers in microbiology · 2016Article
- Comparative genome analysis of Weissella ceti, an emerging pathogen of farm-raised rainbow trout.BMC genomics · 2015Article
- Mechanisms of ribosome rescue in bacteria.Nature reviews. Microbiology · 2015Review
- Small protein B upregulates sensor kinase bvgS expression in Aeromonas veronii.Frontiers in microbiology · 2015Article
- Resolving nonstop translation complexes is a matter of life or death.Journal of bacteriology · 2014Review
- Distinct tmRNA sequence elements facilitate RNase R engagement on rescued ribosomes for selective nonstop mRNA decay.Nucleic acids research · 2014Article
- Trans-translation mediates tolerance to multiple antibiotics and stresses in Escherichia coli.The Journal of antimicrobial chemotherapy · 2013Article
- Active and accurate trans-translation requires distinct determinants in the C-terminal tail of SmpB protein and the mRNA-like domain of transfer messenger RNA (tmRNA).The Journal of biological chemistry · 2013Article
- Small molecule inhibitors of trans-translation have broad-spectrum antibiotic activity.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Through targeted inactivation of the ssrA and smpB genes, we establish that the trans-translation process is necessary for normal growth, adaptation to cellular stress and virulence by the bacterial pathogen Francisella tularensis. The mutant bacteria grow slower, have reduced resistance to heat and cold shocks, and are more sensitive to oxidative stress and sublethal concentrations of antibiotics. Modifications of the tmRNA tag and use of higher-resolution mass spectrometry approaches enabled the identification of a large number of native tmRNA substrates. Of particular significance to understanding the mechanism of trans-translation, we report the discovery of an extended tmRNA tag and extensive ladder-like pattern of endogenous protein-tagging events in F. tularensis that are likely to be a universal feature of tmRNA activity in eubacteria. Furthermore, the structural integrity and the proteolytic function of the tmRNA tag are both crucial for normal growth and virulence of F. tularensis. Significantly, trans-translation mutants of F. tularensis are impaired in replication within macrophages and are avirulent in mouse models of tularemia. By exploiting these attenuated phenotypes, we find that the mutant strains provide effective immune protection in mice against lethal intradermal, intraperitoneal and intranasal challenges with the fully virulent parental strain.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.