ArticlePlant methods2012
The Arabidopsis thaliana-Alternaria brassicicola pathosystem: A model interaction for investigating seed transmission of necrotrophic fungi.
Article in Plant methods, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed.
- Seed-Associated Trichoderma and Their Potential as Biocontrol Agents Against Plant Diseases.Environmental microbiology · 2026Article
- Antimicrobial Peptides as Part of the Arsenal of Constitutive and Inducible Seed Defences in Tomato Seed Exudates Against Pathogens.Molecular plant pathology · 2025Article
- Plant defense compounds can enhance antagonistic effects againstFrontiers in plant science · 2024Article
- Bifunctional role of some biogenic nanoparticles in controlling wilt disease and promoting growth of common bean.AMB Express · 2023Article
- Seed Transmission of Pathogens: Non-Canonical Immune Response inPlants (Basel, Switzerland) · 2022Article
- Identification of stably expressed reference genes for expression studies inFrontiers in plant science · 2022Article
- Article
- Redox-Dependent Structural Modification of Nucleoredoxin Triggers Defense Responses againstInternational journal of molecular sciences · 2020Article
- Role of membrane compartment occupied by Can1 (MCC) and eisosome subdomains in plant pathogenicity of the necrotrophic fungus Alternaria brassicicola.BMC microbiology · 2019Article
- Differences in resource use lead to coexistence of seed-transmitted microbial populations.Scientific reports · 2019Article
- The SCOOP12 peptide regulates defense response and root elongation in Arabidopsis thaliana.Journal of experimental botany · 2019Article
- Responses to Hydric Stress in the Seed-Borne Necrotrophic FungusFrontiers in microbiology · 2019Article
- Genome Sequence of the Necrotrophic Plant PathogenGenome announcements · 2018Article
- Article
- Endoplasmic reticulum stress and fungal pathogenesis.Fungal biology reviews · 2014Article
- Review
- Dehydrin-like proteins in the necrotrophic fungus Alternaria brassicicola have a role in plant pathogenesis and stress response.PloS one · 2013Article
- Role of mannitol metabolism in the pathogenicity of the necrotrophic fungus Alternaria brassicicola.Frontiers in plant science · 2013Article
Corrections and comments
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Authors and funding
10 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundSeed transmission constitutes a major component of the parasitic cycle for several fungal pathogens. However, very little is known concerning fungal or plant genetic factors that impact seed transmission and mechanisms underlying this key biological trait have yet to be clarified. Such lack of available data could be probably explained by the absence of suitable model pathosystem to study plant-fungus interactions during the plant reproductive phase.
resultsHere we report on setting up a new pathosystem that could facilitate the study of fungal seed transmission. Reproductive organs of Arabidopsis thaliana were inoculated with Alternaria brassicicola conidia. Parameters (floral vs fruit route, seed collection date, plant and silique developmental stages) that could influence the seed transmission efficiency were tested to define optimal seed infection conditions. Microscopic observations revealed that the fungus penetrates siliques through cellular junctions, replum and stomata, and into seed coats either directly or through cracks. The ability of the osmosensitive fungal mutant nik1Δ3 to transmit to A. thaliana seeds was analyzed. A significant decrease in seed transmission rate was observed compared to the wild-type parental strain, confirming that a functional osmoregulation pathway is required for efficient seed transmission of the fungus. Similarly, to test the role of flavonoids in seed coat protection against pathogens, a transparent testa Arabidopsis mutant (tt4-1) not producing any flavonoid was used as host plant. Unexpectedly, tt4-1 seeds were infected to a significantly lower extent than wild-type seeds, possibly due to over-accumulation of other antimicrobial metabolites.
conclusionsThe Arabidopsis thaliana-Alternaria brassicicola pathosystem, that have been widely used to study plant-pathogen interactions during the vegetative phase, also proved to constitute a suitable model pathosystem for detailed analysis of plant-pathogen interactions during the reproductive phase. We demonstrated that it provides an excellent system for investigating the impact of different fungal or plant mutations on the seed transmission process and therefore paves the way towards future high-throughput screening of both Arabidopsis and fungal mutant.
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