ArticlePloS one2012
Co-stimulation of PAFR and CD36 is required for oxLDL-induced human macrophages activation.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 51 citations in OpenAlex.
- Bixin, a New Atheroprotective Carotenoid Candidate, Prevents oxLDL-Induced Cytotoxicity and Mitochondrial Dysfunction in Macrophages: Involvement of the Nrf2 and NF-κB Pathways.Foods (Basel, Switzerland) · 2024Article
- Gsα Regulates Macrophage Foam Cell Formation During Atherosclerosis.Circulation research · 2024Article
- PI3K signaling promotes formation of lipid-laden foamy macrophages at the spinal cord injury site.Neurobiology of disease · 2024Article
- Lipids and lipoproteins may play a role in the neuropathology of Alzheimer's disease.Frontiers in neuroscience · 2023Review
- PAF signaling plays a role in obesity-induced adipose tissue remodeling.International journal of obesity (2005) · 2022Article
- Matrix stiffness modulates hepatic stellate cell activation into tumor-promoting myofibroblasts via E2F3-dependent signaling and regulates malignant progression.Cell death & disease · 2021Article
- The Metaflammatory and Immunometabolic Role of Macrophages and Microglia in Diabetic Retinopathy.Human cell · 2021Review
- Role of PI3K in the Progression and Regression of Atherosclerosis.Frontiers in pharmacology · 2021Review
- Article
- Oxidized Low-Density Lipoprotein Induces WNT5A Signaling Activation in THP-1 Derived Macrophages and a Human Aortic Vascular Smooth Muscle Cell Line.Frontiers in cardiovascular medicine · 2020Article
- Isolation, synthesis and bioactivity studies of phomactin terpenoids.Nature chemistry · 2018Article
- Methionine oxidized apolipoprotein A-I at the crossroads of HDL biogenesis and amyloid formation.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2018Article
- Boosting Adaptive Immunity: A New Role for PAFR Antagonists.Scientific reports · 2016Article
- Curcumin inhibits monocyte chemoattractant protein-1 expression and enhances cholesterol efflux by suppressing the c-Jun N-terminal kinase pathway in macrophage.Inflammation research : official journal of the European Histamine Research Society ... [et al.] · 2014Article
- MyD88-dependent interplay between myeloid and endothelial cells in the initiation and progression of obesity-associated inflammatory diseases.The Journal of experimental medicine · 2014Article
- Activation of platelet-activating factor receptor exacerbates renal inflammation and promotes fibrosis.Laboratory investigation; a journal of technical methods and pathology · 2014Article
- HIV-1 Nef impairs key functional activities in human macrophages through CD36 downregulation.PloS one · 2014Article
- CD36-specific antibodies block release of HIV-1 from infected primary macrophages and its transmission to T cells.The Journal of experimental medicine · 2013Article
- Adipose tissue insulin sensitivity and macrophage recruitment: Does PI3K pick the pathway?Adipocyte · 2013Article
- Clearance of apoptotic cells by macrophages induces regulatory phenotype and involves stimulation of CD36 and platelet-activating factor receptor.Mediators of inflammation · 2013Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The oxidative process of LDL particles generates molecules which are structurally similar to platelet-activating factor (PAF), and some effects of oxidized LDL (oxLDL) have been shown to be dependent on PAF receptor (PAFR) activation. In a previous study, we showed that PAFR is required for upregulation of CD36 and oxLDL uptake. In the present study we analyzed the molecular mechanisms activated by oxLDL in human macrophages and the contribution of PAFR to this response. Human adherent monocytes/macrophages were stimulated with oxLDL. Uptake of oxLDL and CD36 expression were determined by flow cytometry; MAP kinases and Akt phosphorylation by Western blot; IL-8 and MCP-1 concentration by ELISA and mRNA expression by real-time PCR. To investigate the participation of the PI3K/Akt pathway, Gαi-coupled protein or PAFR, macrophages were treated with LY294002, pertussis toxin or with the PAFR antagonists WEB2170 and CV3988, respectively before addition of oxLDL. It was found that the addition of oxLDL to human monocytes/macrophages activates the PI3K/Akt pathway which in turn activates the MAPK (p38 and JNK). Phosphorylation of Akt requires the engagement of PAFR and a Gαi-coupled protein. The upregulation of CD36 protein and the uptake of oxLDL as well as the IL-8 production are dependent on PI3K/Akt pathway activation. The increased CD36 protein expression is dependent on PAFR and Gαi-coupled protein. Transfection studies using HEK 293t cells showed that oxLDL uptake occurs with either PAFR or CD36, but IL-8 production requires the co-transfection of both PAFR and CD36. These findings show that PAFR has a pivotal role in macrophages response to oxLDL and suggest that pharmacological intervention at the level of PAFR activation might be beneficial in atherosclerosis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.