ArticlePloS one2012
Protective activity of Streptococcus pneumoniae Spr1875 protein fragments identified using a phage displayed genomic library.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 31 citations in OpenAlex.
- Characterization of VldE (Spr1875), a Pneumococcal Two-State l,d-Endopeptidase with a Four-Zinc Cluster in the Active Site.ACS catalysis · 2024Article
- Bacteriophage-based bioassays: an expected paradigm shift in microbial diagnostics.Future microbiology · 2024Review
- Neutrophils discriminate live from dead bacteria by integrating signals initiated by Fprs and TLRs.The EMBO journal · 2022Article
- IL-10-producing NK cells exacerbate sublethal Streptococcus pneumoniae infection in the lung.Translational research : the journal of laboratory and clinical medicine · 2020Article
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- Review
- The plasminogen binding protein PbsP is required for brain invasion by hypervirulent CC17 Group B streptococci.Scientific reports · 2018Article
- Functional characterization of a monoclonal antibody epitope using a lambda phage display-deep sequencing platform.Scientific reports · 2016Article
- Neutrophils Directly Recognize Group B Streptococci and Contribute to Interleukin-1β Production during Infection.PloS one · 2016Article
- The interleukin-1β/CXCL1/2/neutrophil axis mediates host protection against group B streptococcal infection.Infection and immunity · 2014Article
- FbsC, a novel fibrinogen-binding protein, promotes Streptococcus agalactiae-host cell interactions.The Journal of biological chemistry · 2014Article
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- Identification of potential new protein vaccine candidates through pan-surfomic analysis of pneumococcal clinical isolates from adults.PloS one · 2013Article
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- Article
Corrections and comments
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Authors and funding
16 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
There is considerable interest in pneumococcal protein antigens capable of inducing serotype-independent immunoprotection and of improving, thereby, existing vaccines. We report here on the immunogenic properties of a novel surface antigen encoded by ORF spr1875 in the R6 strain genome. An antigenic fragment encoded by spr1875, designated R4, was identified using a Streptococcus pneumoniae phage displayed genomic library after selection with a human convalescent serum. Immunofluorescence analysis with anti-R4 antisera showed that Spr1875 was expressed on the surface of strains belonging to different serotypes. Moreover, the gene was present with little sequence variability in 27 different pneumococcal strains isolated worldwide. A mutant lacking Spr1875 was considerably less virulent than the wild type D39 strain in an intravenous mouse model of infection. Moreover, immunization with the R4 recombinant fragment, but not with the whole Spr1875 protein, induced significant protection against sepsis in mice. Lack of protection after immunization with the whole protein was related to the presence of immunodominant, non-protective epitopes located outside of the R4 fragment. In conclusion, our data indicate that Spr1875 has a role in pneumococcal virulence and is immunogenic. As the R4 fragment conferred immunoprotection from experimental sepsis, selected antigenic fragments of Spr1875 may be useful for the development of a pneumococcal protein-based vaccine.
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