ArticlePLoS genetics2012
Downregulation of chloroplast RPS1 negatively modulates nuclear heat-responsive expression of HsfA2 and its target genes in Arabidopsis.
Article in PLoS genetics, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 67 papers.
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Who cites it
67 citing papers in PubMed, 129 citations in OpenAlex.
- Design Principles of Plant Thermotolerance: Integrating Photosynthesis, Reproduction, and Field Resilience in a Warming Climate.Plant, cell & environment · 2026Review
- More than the 'master regulator of cytosolic proteostasis': new roles for HSF1 in promoting tissue health.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2026Review
- A Review of Nuclear-Organellar Communication in Response to Heat Stress.Plants (Basel, Switzerland) · 2026Review
- Integrating molecular networks and physiological adaptation for heat-resilient crops.Journal of integrative plant biology · 2026Review
- Physiological and multi-omics insights into ultraviolet B-induced stress adaptation in Fritillaria cirrhosa native to the Qinghai-Tibet Plateau.Journal of advanced research · 2026Article
- ClBCM: an EMS mutated gene regulating chlorophyll synthesis and adaptation to diverse stresses in watermelon.Molecular horticulture · 2026Article
- Maturase K forms a plastidial splicing complex with a neofunctionalized branching enzyme.Nature communications · 2026Article
- Biogenic retrograde signaling via GUN1 ensures thermotolerant chloroplast biogenesis during seedling establishment in Arabidopsis thaliana.Journal of integrative plant biology · 2026Article
- Proteostasis and protein quality control in chloroplasts: mechanisms and novel insights related to protein mislocalization.Journal of experimental botany · 2025Review
- Genome-Wide Characterization of the Heat Shock Transcription Factor Gene Family inCurrent issues in molecular biology · 2025Article
- Review
- Haplotype-resolved genome ofHorticulture research · 2024Article
- The first two whole mitochondrial genomes for the genus Dactylis species: assembly and comparative genomics analysis.BMC genomics · 2024Article
- Regulation of chloroplast biogenesis, development, and signaling by endogenous and exogenous cues.Physiology and molecular biology of plants : an international journal of functional plant biology · 2024Review
- Comparative transcriptome analysis reveals nicotine metabolism is a critical component for enhancing stress response intensity of innate immunity system in tobacco.Frontiers in plant science · 2024Article
- iTRAQ-based proteomic analysis reveals the effect of ribosomal proteins on essential-oil accumulation inPeerJ · 2024Article
- Rice HEAT SHOCK PROTEIN60-3B maintains male fertility under high temperature by starch granule biogenesis.Plant physiology · 2023Article
- Perturbation of protein homeostasis brings plastids at the crossroad between repair and dismantling.PLoS genetics · 2023Article
- Chloroplast Pan-Genomes and Comparative Transcriptomics Reveal Genetic Variation and Temperature Adaptation in the Cucumber.International journal of molecular sciences · 2023Article
- Global Analysis of Dark- and Heat-Regulated Alternative Splicing in Arabidopsis.International journal of molecular sciences · 2023Article
7 more citing papers are in PubMed but not listed here.
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Authors and funding
8 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Heat stress commonly leads to inhibition of photosynthesis in higher plants. The transcriptional induction of heat stress-responsive genes represents the first line of inducible defense against imbalances in cellular homeostasis. Although heat stress transcription factor HsfA2 and its downstream target genes are well studied, the regulatory mechanisms by which HsfA2 is activated in response to heat stress remain elusive. Here, we show that chloroplast ribosomal protein S1 (RPS1) is a heat-responsive protein and functions in protein biosynthesis in chloroplast. Knockdown of RPS1 expression in the rps1 mutant nearly eliminates the heat stress-activated expression of HsfA2 and its target genes, leading to a considerable loss of heat tolerance. We further confirm the relationship existed between the downregulation of RPS1 expression and the loss of heat tolerance by generating RNA interference-transgenic lines of RPS1. Consistent with the notion that the inhibited activation of HsfA2 in response to heat stress in the rps1 mutant causes heat-susceptibility, we further demonstrate that overexpression of HsfA2 with a viral promoter leads to constitutive expressions of its target genes in the rps1 mutant, which is sufficient to reestablish lost heat tolerance and recovers heat-susceptible thylakoid stability to wild-type levels. Our findings reveal a heat-responsive retrograde pathway in which chloroplast translation capacity is a critical factor in heat-responsive activation of HsfA2 and its target genes required for cellular homeostasis under heat stress. Thus, RPS1 is an essential yet previously unknown determinant involved in retrograde activation of heat stress responses in higher plants.
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