ArticleProceedings of the National Academy of Sciences of the United States of America2012
Activation of 5'-3' exoribonuclease Xrn1 by cofactor Dcs1 is essential for mitochondrial function in yeast.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 36 citations in OpenAlex.
- Perturbation of RNA homeostasis impairs mitochondrial respiration during poxvirus infection through excess RNA accumulation.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- The Functional Meaning of 5'UTR in Protein-Coding Genes.International journal of molecular sciences · 2023Review
- Association of ScV-LA Virus with Host Protein Metabolism Determined by Proteomics Analysis and Cognate RNA Sequencing.Viruses · 2022Article
- Adaptive Response ofJournal of fungi (Basel, Switzerland) · 2022Article
- mRNA-Decapping Associated DcpS Enzyme Controls Critical Steps of Neuronal Development.Cerebral cortex (New York, N.Y. : 1991) · 2022Article
- Xrn1 is a deNADding enzyme modulating mitochondrial NAD-capped RNA.Nature communications · 2022Article
- Host Genetic Risk Factors for Chlamydia trachomatis-Related Infertility in Women.The Journal of infectious diseases · 2021Article
- Exome resequencing and GWAS for growth, ecophysiology, and chemical and metabolomic composition of wood of Populus trichocarpa.BMC genomics · 2019Article
- Beet Necrotic Yellow Vein Virus Noncoding RNA Production Depends on a 5'→3' Xrn Exoribonuclease Activity.Viruses · 2018Article
- Article
- Small Molecules in Development for the Treatment of Spinal Muscular Atrophy.Journal of medicinal chemistry · 2016Review
- An excess of catalytically required motions inhibits the scavenger decapping enzyme.Nature chemical biology · 2015Article
- DcpS is a transcript-specific modulator of RNA in mammalian cells.RNA (New York, N.Y.) · 2015Article
- Mutations in DCPS and EDC3 in autosomal recessive intellectual disability indicate a crucial role for mRNA decapping in neurodevelopment.Human molecular genetics · 2015Article
- Elimination of cap structures generated by mRNA decay involves the new scavenger mRNA decapping enzyme Aph1/FHIT together with DcpS.Nucleic acids research · 2015Article
- Interaction between the poly(A)-binding protein Pab1 and the eukaryotic release factor eRF3 regulates translation termination but not mRNA decay in Saccharomyces cerevisiae.RNA (New York, N.Y.) · 2015Article
- Coupling mRNA synthesis and decay.Molecular and cellular biology · 2014Review
- Quality control of assembly-defective U1 snRNAs by decapping and 5'-to-3' exonucleolytic digestion.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- An evolutionarily conserved RNase-based mechanism for repression of transcriptional positive autoregulation.Molecular microbiology · 2014Article
- The decapping scavenger enzyme DCS-1: a new modulator of miRNA turnover.Cell cycle (Georgetown, Tex.) · 2013Article
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Authors and funding
5 authors at 5 institutions in 2 countries.
Funding
Abstract
The scavenger decapping enzyme Dcs1 has been shown to facilitate the activity of the cytoplasmic 5'-3' exoribonuclease Xrn1 in eukaryotes. Dcs1 has also been shown to be required for growth in glycerol medium. We therefore wondered whether the capacity to activate RNA degradation could account for its requirement for growth on this carbon source. Indeed, a catalytic mutant of Xrn1 is also unable to grow in glycerol medium, and removal of the nuclear localization signal of Rat1, the nuclear homolog of Xrn1, restores glycerol growth. A cytoplasmic 5'-3' exoribonuclease activity is therefore essential for yeast growth on glycerol, suggesting that Xrn1 activation by Dcs1 is physiologically important. In fact, Xrn1 is essentially inactive in the absence of Dcs1 in vivo. We analyzed the role of Dcs1 in the control of exoribonuclease activity in vitro and propose that Dcs1 is a specific cofactor of Xrn1. Dcs1 does not stimulate the activity of other 5'-3' exoribonucleases, such as Rat1, in vitro. We demonstrate that Dcs1 improves the apparent affinity of Xrn1 for RNA and that Xrn1 and Dcs1 can form a complex in vitro. We examined the biological significance of this regulation by performing 2D protein gel analysis. We observed that a set of proteins showing decreased levels in a DCS deletion strain, some essential for respiration, are also systematically decreased in an XRN1 deletion mutant. Therefore, we propose that the activation of Xrn1 by Dcs1 is important for respiration.
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