ArticleThe Plant cell2012
Arabidopsis microtubule-associated protein MAP65-3 cross-links antiparallel microtubules toward their plus ends in the phragmoplast via its distinct C-terminal microtubule binding domain.
Article in The Plant cell, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed.
- Progressive oxygenation of developing leaves directs morphogenesis.Science advances · 2026Article
- IMK2-IMK3 module regulates biogenesis of nascent cell walls and postcytokinetic differentiation inScience advances · 2026Article
- Thyme Oil Alleviates Cadmium-Induced Disturbances in Mitotic Activity, Cytoskeletal Organization and H3T3/H3S10 Phosphorylation inInternational journal of molecular sciences · 2026Article
- The tail domain of the plant kinesin-12 POK2 is a versatile interaction hub.Journal of cell science · 2025Article
- Cell Division Imaging of Tobacco BY-2 Cells in a 3D Volume by Two-Photon Spinning-Disk Confocal Microscopy.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Arabidopsis α-Aurora kinase plays a role in cytokinesis through regulating MAP65-3 association with microtubules at phragmoplast midzone.Nature communications · 2024Article
- Comprehensive analyses of microtubule-associated protein MAP65 family genes in Cucurbitaceae and CsaMAP65s expression profiles in cucumber.Journal of applied genetics · 2023Article
- The phosphoinositide signature guides the final step of plant cytokinesis.Science advances · 2023Article
- Enhancing health-promoting isothiocyanates in Chinese kale sprouts via manipulatingHorticulture research · 2023Article
- Microtubule Regulation in Plants: From Morphological Development to Stress Adaptation.Biomolecules · 2023Review
- Overlapping Localization of MAP65-2, -6, and -7 in Arabidopsis Hypocotyl Cells.microPublication biology · 2023Article
- Defects in division plane positioning in the root meristematic zone affect cell organization in the differentiation zone.Journal of cell science · 2022Article
- Cell biology of primary cell wall synthesis in plants.The Plant cell · 2022Review
- Rice microtubule-associated protein OsMAP65-3.1, but not OsMAP65-3.2, plays a critical role in phragmoplast microtubule organization in cytokinesis.Frontiers in plant science · 2022Article
- Mechanical stress effects on transcriptional regulation of genes encoding microtubule- and actin-associated proteins.Physiology and molecular biology of plants : an international journal of functional plant biology · 2022Article
- The Protein Phosphatase PP2A Plays Multiple Roles in Plant Development by Regulation of Vesicle Traffic-Facts and Questions.International journal of molecular sciences · 2021Review
- CDKD-dependent activation of CDKA;1 controls microtubule dynamics and cytokinesis during meiosis.The Journal of cell biology · 2020Article
- TANGLED1 mediates microtubule interactions that may promote division plane positioning in maize.The Journal of cell biology · 2020Article
- Complementary Superresolution Visualization of Composite Plant Microtubule Organization and Dynamics.Frontiers in plant science · 2020Article
- Comprehensive characterization of a floral mutant reveals the mechanism of hooked petal morphogenesis in Chrysanthemum morifolium.Plant biotechnology journal · 2019Article
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Authors and funding
5 authors.
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Abstract
Plant cytokinesis is brought about by the phragmoplast, which contains an antiparallel microtubule (MT) array. The MT-associated protein MAP65-3 acts as an MT-bundling factor that specifically cross-links antiparallel MTs near their plus ends. MAP65 family proteins contain an N-terminal dimerization domain and C-terminal MT interaction domain. Compared with other MAP65 isoforms, MAP65-3 contains an extended C terminus. A MT binding site was discovered in the region between amino acids 496 and 588 and found to be essential for the organization of phragmoplast MTs. The frequent cytokinetic failure caused by loss of MAP65-3 was not rescued by ectopic expression of MAP65-1 under the control of the MAP65-3 promoter, indicating nonoverlapping functions between the two isoforms. In the presence of MAP65-3, however, ectopic MAP65-1 appeared in the phragmoplast midline. We show that MAP65-1 could acquire the function of MAP65-3 when the C terminus of MAP65-3, which contains the MT binding site, was grafted to it. Our results also show that MAP65-1 and MAP65-3 may share redundant functions in MT stabilization. Such a stabilization effect was likely brought about by MT binding and bundling. We conclude that MAP65-3 contains a distinct C-terminal MT binding site with a specific role in cross-linking antiparallel MTs toward their plus ends in the phragmoplast.
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