ArticleThe Plant cell2012
The Golgi-localized Arabidopsis endomembrane protein12 contains both endoplasmic reticulum export and Golgi retention signals at its C terminus.
Article in The Plant cell, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 58 papers.
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58 citing papers in PubMed, 101 citations in OpenAlex.
- Trafficking and localization of Golgi-residentFrontiers in plant science · 2025Review
- Toxin Homology Domain in Plant Type 2 Prolyl 4-Hydroxylases Acts as a Golgi Localization Domain.Cells · 2024Article
- Multi-Omics Analysis of a Chromosome Segment Substitution Line Reveals a New Regulation Network for Soybean Seed Storage Profile.International journal of molecular sciences · 2024Article
- Arabidopsis TETRASPANIN8 mediates exosome secretion and glycosyl inositol phosphoceramide sorting and trafficking.The Plant cell · 2024Article
- An Overview of Protein Secretion in Plant Cells.Methods in molecular biology (Clifton, N.J.) · 2024Review
- The plant-unique protein DRIF1 coordinates with sorting nexin 1 to regulate membrane protein homeostasis.The Plant cell · 2023Article
- A new method for studying the orientation of membrane proteins in plants based on the release of a fluorescent protein tag by TEV protease.Plant communications · 2023Article
- VAMP724 and VAMP726 are involved in autophagosome formation inAutophagy · 2023Article
- Application of a novel strong promoter from Chinese fir (Frontiers in plant science · 2023Article
- A Golgi-Located Transmembrane Nine Protein Gene TMN11 Functions in Manganese/Cadmium Homeostasis and Regulates Growth and Seed Development in Rice.International journal of molecular sciences · 2022Article
- The plant ESCRT component FREE1 regulates peroxisome-mediated turnover of lipid droplets in germinating Arabidopsis seedlings.The Plant cell · 2022Article
- Tip growth defective1 interacts with cellulose synthase A3 to regulate cellulose biosynthesis in Arabidopsis.Plant molecular biology · 2022Article
- Characterization ofFrontiers in plant science · 2022Article
- SNAREs Regulate Vesicle Trafficking During Root Growth and Development.Frontiers in plant science · 2022Review
- An Update on the Key Factors Required for Plant Golgi Structure Maintenance.Frontiers in plant science · 2022Review
- Motif-based endomembrane trafficking.Plant physiology · 2021Article
- Golgi-localized membrane protein AtTMN1/EMP12 functions in the deposition of rhamnogalacturonan II and I for cell growth in Arabidopsis.Journal of experimental botany · 2021Article
- Go your own way: membrane-targeting sequences.Plant physiology · 2021Article
- The FUSED LEAVES1-ADHERENT1 regulatory module is required for maize cuticle development and organ separation.The New phytologist · 2021Article
- p24 Family Proteins Are Involved in Transport to the Plasma Membrane of GPI-Anchored Proteins in Plants.Plant physiology · 2020Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Endomembrane proteins (EMPs), belonging to the evolutionarily conserved transmembrane nine superfamily in yeast and mammalian cells, are characterized by the presence of a large lumenal N terminus, nine transmembrane domains, and a short cytoplasmic tail. The Arabidopsis thaliana genome contains 12 EMP members (EMP1 to EMP12), but little is known about their protein subcellular localization and function. Here, we studied the subcellular localization and targeting mechanism of EMP12 in Arabidopsis and demonstrated that (1) both endogenous EMP12 (detected by EMP12 antibodies) and green fluorescent protein (GFP)-EMP12 fusion localized to the Golgi apparatus in transgenic Arabidopsis plants; (2) GFP fusion at the C terminus of EMP12 caused mislocalization of EMP12-GFP to reach post-Golgi compartments and vacuoles for degradation in Arabidopsis cells; (3) the EMP12 cytoplasmic tail contained dual sorting signals (i.e., an endoplasmic reticulum export motif and a Golgi retention signal that interacted with COPII and COPI subunits, respectively); and (4) the Golgi retention motif of EMP12 retained several post-Golgi membrane proteins within the Golgi apparatus in gain-of-function analysis. These sorting signals are highly conserved in all plant EMP isoforms and, thus, likely represent a general mechanism for EMP targeting in plant cells.
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