ArticleMolecules and cells2012
Bacterial transposons are co-transferred with T-DNA to rice chromosomes during Agrobacterium-mediated transformation.
Article in Molecules and cells, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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10 citing papers in PubMed.
- Editing theInternational journal of molecular sciences · 2025Article
- Effects of different light conditions on transient expression and biomass inOpen life sciences · 2023Article
- Issue when expressing a recombinant protein under the control of pFrontiers in plant science · 2023Article
- Efficient CRISPR-mediated base editing inProceedings of the National Academy of Sciences of the United States of America · 2021Article
- Maize Transformation: From Plant Material to the Release of Genetically Modified and Edited Varieties.Frontiers in plant science · 2021Review
- Genetic Modification for Wheat Improvement: From Transgenesis to Genome Editing.BioMed research international · 2019Review
- An Insight into T-DNA Integration Events in Medicago sativa.International journal of molecular sciences · 2017Article
- The widely used Nicotiana benthamiana 16c line has an unusual T-DNA integration pattern including a transposon sequence.PloS one · 2017Article
- Whole-genome analysis of herbicide-tolerant mutant rice generated by Agrobacterium-mediated gene targeting.Plant & cell physiology · 2015Article
- A whole-genome analysis of a transgenic rice seed-based edible vaccine against cedar pollen allergy.DNA research : an international journal for rapid publication of reports on genes and genomes · 2013Article
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2 authors.
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Abstract
Agrobacterium tumefaciens is widely utilized for delivering a foreign gene into a plant's genome. We found the bacterial transposon Tn5393 in transgenic rice plants. Analysis of the flanking sequences of the transferred-DNA (T-DNA) identified that a portion of the Tn5393 sequence was present immediately next to the end of the T-DNA. Because this transposon was present in A. tumefaciens strain LBA4404, but not in EHA105 and GV3101, our findings indicated that Tn5393 was transferred from LBA4404 into the rice genome during the transformation process. We also noted that another bacterial transposon, Tn5563, is present in transgenic plants. Analyses of 331 transgenic lines revealed that 26.0% carried Tn5393 and 2.1% contained Tn5563. In most of the lines, an intact transposon was integrated into the T-DNA and transferred to the rice chromosome. More than one copy of T-DNA was introduced into the plants, often at a single locus. This resulted in T-DNA repeats of normal and transposon-carrying TDNA that generated deletions of a portion of the T-DNA, joining the T-DNA end to the bacterial transposon. Based on these data, we suggest that one should carefully select the appropriate Agrobacterium strain to avoid undesirable transformation of such sequences.
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