ArticleMolecular and biochemical parasitology2012
Importance of enolase in Giardia lamblia differentiation.
Article in Molecular and biochemical parasitology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 17 citations in OpenAlex.
- Therapeutic and vaccinomic potential of moonlighting proteins for the discovery and design of drugs and vaccines against schistosomiasis.American journal of translational research · 2024Review
- Conserved metabolic enzymes as vaccine antigens for giardiasis.PLoS neglected tropical diseases · 2022Article
- Article
- Click chemistry-facilitated comprehensive identification of proteins adducted by antimicrobial 5-nitroimidazoles for discovery of alternative drug targets against giardiasis.PLoS neglected tropical diseases · 2020Article
- Identification of Conserved Candidate Vaccine Antigens in the Surface Proteome of Giardia lamblia.Infection and immunity · 2019Article
- Giardia lamblia: identification of molecules that contribute to direct mast cell activation.Parasitology research · 2018Article
- Serodiagnostic Potential of Alpha-Enolase FromFrontiers in microbiology · 2018Article
- Immunolocation and enzyme activity analysis of Cryptosporidium parvum enolase.Parasites & vectors · 2017Article
- Carbon partitioning in green algae (chlorophyta) and the enolase enzyme.Metabolites · 2014Article
- The proteome landscape of Giardia lamblia encystation.PloS one · 2013Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
The ability of Giardia to differentiate into cysts which survive in the environment and release the virulent trophozoites after ingestion in the small intestine is essential for transmission and disease. We examined the role of enolase, a glycolytic enzyme, in Giardia differentiation. The sequence of Giardia lamblia enolase (gEno) is most similar to enolases in Homo sapiens and Leishmania mexicana, and shows the conserved catalytic and metal-binding residues. We used an integration vector to stably express wild type and mutant gEno. In trophozoites, wild type gEno localized to the cell membrane, caudal flagella and cytosol. gEno is present on the wall of mature cysts, but not in encystation secretory vesicles (ESV). The expression of gEno with a deletion of residues G167-K169, or mutations H389Q/R390S significantly inhibited excystation while mutation of residue D257K had no effect. These results suggest a role for enolase in regulation of Giardia excystation.
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