ArticleCellular microbiology2012
The profile of snoRNA-derived microRNAs that regulate expression of variant surface proteins in Giardia lamblia.
Article in Cellular microbiology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 31 citations in OpenAlex.
- miR-375 Regulates Extracellular Vesicle Secretion From Giardia duodenalis via Targeting Rab1a.Transboundary and emerging diseases · 2026Article
- miRNA, New Perspective to World of Intestinal Protozoa and Toxoplasma.Acta parasitologica · 2024Review
- Exploration ofParasitology · 2024Review
- MicroRNAs: master regulators in host-parasitic protist interactions.Open biology · 2022Review
- The origin of exosomal miR-1246 in human cancer cells.RNA biology · 2019Article
- Giardia lamblia miRNAs as a new diagnostic tool for human giardiasis.PLoS neglected tropical diseases · 2019Article
- Nucleomorph Small RNAs in Cryptophyte and Chlorarachniophyte Algae.Genome biology and evolution · 2019Article
- Comprehensive Evolutionary Analysis of the Major RNA-Induced Silencing Complex Members.Scientific reports · 2018Article
- Article
- Promoter-based identification of novel non-coding RNAs reveals the presence of dicistronic snoRNA-miRNA genes in Arabidopsis thaliana.BMC genomics · 2015Article
- Assessing the gene regulatory properties of Argonaute-bound small RNAs of diverse genomic origin.Nucleic acids research · 2015Article
- Both endo-siRNAs and tRNA-derived small RNAs are involved in the differentiation of primitive eukaryote Giardia lamblia.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- The microRNAs in an ancient protist repress the variant-specific surface protein expression by targeting the entire coding sequence.PLoS pathogens · 2014Article
- Insights into snoRNA biogenesis and processing from PAR-CLIP of snoRNA core proteins and small RNA sequencing.Genome biology · 2013Article
- Article
- Global proteomic analysis of the oocyst/sporozoite of Toxoplasma gondii reveals commitment to a host-independent lifestyle.BMC genomics · 2013Article
- Transition of a microRNA from repressing to activating translation depending on the extent of base pairing with the target.PloS one · 2013Article
- DNA topoisomerase II is involved in regulation of cyst wall protein genes and differentiation in Giardia lamblia.PLoS neglected tropical diseases · 2013Article
- Processing of snoRNAs as a new source of regulatory non-coding RNAs: snoRNA fragments form a new class of functional RNAs.BioEssays : news and reviews in molecular, cellular and developmental biology · 2013Review
- New perspectives on the diversification of the RNA interference system: insights from comparative genomics and small RNA sequencing.Wiley interdisciplinary reviews. RNAReview
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
In the current investigation, we analysed all the known small nucleolar RNAs (snoRNAs) in the deeply branching protozoan parasite Giardia lamblia for potential microRNAs (miRNAs) that might be derived from them. Two putative miRNAs have since been identified by Northern blot, primer extension, 3' RACE and co-immunoprecipitation with Giardia Argonaute (GlAgo), and designated miR6 and miR10. Giardia Dicer (GlDcr) is capable of processing the snoRNAs into the corresponding miRNAs in vitro. Potential miR6 and miR10 binding sites in Giardia genome were predicted bio-informatically. A miR6 binding site was found at the 3' untranslated regions (UTR) of 44 variant surface protein (vsp) genes, whereas a miR10 binding site was identified at the 3' end of 159 vsp open-reading frames. Thirty-three of these vsp genes turned out to contain binding sites for both miR6 and miR10. A reporter mRNA tagged with the 3' end of vsp1267, which contains the target sites for both miRNAs, was translationally repressed by both miRNAs in Giardia. Episomal expression of an N-terminal c-myc tagged VSP1267 was found significantly repressed by introducing either miR6 or miR10 into the cells and the repressive effects were additive. When the 2'-O-methyl antisense oligos (ASOs) of either miR6 or miR10 was introduced, however, there was an enhancement of tagged VSP1267 expression suggesting an inhibition of the repressive effects of endogenous miR6 or miR10 by the ASOs. Of the total 220 vsp genes in Giardia, we have now found 178 of them carrying putative binding sites for all the miRNAs that have been currently identified, suggesting that miRNAs are likely the regulators of VSP expression in Giardia.
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