ArticleGenetics research international2011
Comparative Study of Cyanobacterial and E. coli RNA Polymerases: Misincorporation, Abortive Transcription, and Dependence on Divalent Cations.
Article in Genetics research international, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
What it found
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Who cites it
16 citing papers in PubMed, 22 citations in OpenAlex.
- Structure and function of the Si3 insertion integrated into the trigger loop/helix of cyanobacterial RNA polymerase.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Structure and function of the Si3 insertion integrated into the trigger loop/helix of cyanobacterial RNA polymerase.bioRxiv : the preprint server for biology · 2024Article
- Control of Transcription Initiation by Biased Thermal Fluctuations on Repetitive Genomic Sequences.Biomolecules · 2020Article
- Genome-Wide Analysis of RNA Decay in the CyanobacteriummSystems · 2020Article
- High intrinsic hydrolytic activity of cyanobacterial RNA polymerase compensates for the absence of transcription proofreading factors.Nucleic acids research · 2020Article
- Structural Basis of Transcription: RNA Polymerase Backtracking and Its Reactivation.Molecular cell · 2019Article
- The Role of Pyrophosphorolysis in the Initiation-to-Elongation Transition by E. coli RNA Polymerase.Journal of molecular biology · 2019Article
- Synthesis of 2'-Fluoro RNA by Syn5 RNA polymerase.Nucleic acids research · 2015Article
- Design and analysis of LacI-repressed promoters and DNA-looping in a cyanobacterium.Journal of biological engineering · 2014Article
- Transcription elongation. Heterogeneous tracking of RNA polymerase and its biological implications.Transcription · 2014Review
- Engineered transcriptional systems for cyanobacterial biotechnology.Frontiers in bioengineering and biotechnology · 2014Review
- Direct assessment of transcription fidelity by high-resolution RNA sequencing.Nucleic acids research · 2013Article
- The RNA polymerase of marine cyanophage Syn5.The Journal of biological chemistry · 2013Article
- Basic mechanism of transcription by RNA polymerase II.Biochimica et biophysica acta · 2013Review
- Defining the divergent enzymatic properties of RNA polymerases I and II.The Journal of biological chemistryArticle
- Regulatory Tools for Controlling Gene Expression in Cyanobacteria.Advances in experimental medicine and biologyReview
Corrections and comments
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Authors and funding
3 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
If Mg(2+) ion is replaced by Mn(2+) ion, RNA polymerase tends to misincorporate noncognate nucleotide, which is thought to be one of the reasons for the toxicity of Mn(2+) ion. Therefore, most cells have Mn(2+) ion at low intracellular concentrations, but cyanobacteria need the ion at a millimolar concentration to maintain photosynthetic machinery. To analyse the mechanism for resistance against the abundant Mn(2+) ion, we compared the properties of cyanobacterial and E. coli RNA polymerases. The cyanobacterial enzyme showed a lower level of abortive transcription and less misincorporation than the E. coli enzyme. Moreover, the cyanobacterial enzyme showed a slower rate of the whole elongation by an order of magnitude, paused more frequently, and cleaved its transcript faster in the absence of NTPs. In conclusion, cyanobacterial RNA polymerase maintains the fidelity of transcription against Mn(2+) ion by deliberate incorporation of a nucleotide at the cost of the elongation rate. The cyanobacterial and the E. coli enzymes showed different sensitivities to Mg(2+) ion, and the physiological role of the difference is also discussed.
Identifiers
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.