ArticleStem cells international2012
Small molecules greatly improve conversion of human-induced pluripotent stem cells to the neuronal lineage.
Article in Stem cells international, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
What it found
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Who cites it
29 citing papers in PubMed, 65 citations in OpenAlex.
- Human-Induced Pluripotent Stem Cell (iPSC)-Derived GABAergic Neuron Differentiation in Bipolar Disorder.Cells · 2024Article
- From 2D to 3D: Development of Monolayer Dopaminergic Neuronal and Midbrain Organoid Cultures for Parkinson's Disease Modeling and Regenerative Therapy.International journal of molecular sciences · 2023Review
- Modelling ciliopathy phenotypes in human tissues derived from pluripotent stem cells with genetically ablated cilia.Nature biomedical engineering · 2022Article
- Epigenetic regulation during human cortical development: Seq-ing answers from the brain to the organoid.Neurochemistry international · 2021Review
- Patterning inconsistencies restrict the true potential of dopaminergic neurons derived from human induced pluripotent stem cells.Neural regeneration research · 2021Article
- Article
- Neuronal Trans-differentiation by Transcription Factors Ascl1 and Nurr1: Induction of a Dopaminergic Neurotransmitter Phenotype in Cortical GABAergic Neurons.Molecular neurobiology · 2020Article
- A compendious summary of Parkinson's disease patient-derived iPSCs in the first decade.Annals of translational medicine · 2019Review
- G2019S Variation in LRRK2: An Ideal Model for the Study of Parkinson's Disease?Frontiers in human neuroscience · 2019Review
- Highly efficient methods to obtain homogeneous dorsal neural progenitor cells from human and mouse embryonic stem cells and induced pluripotent stem cells.Stem cell research & therapy · 2018Article
- In Vitro Differentiation of Neural-Like Cells from Human Embryonic Stem Cells by A Combination of Dorsomorphin, XAV939, and A8301.Cell journal · 2018Article
- RNA stores tau reversibly in complex coacervates.PLoS biology · 2017Article
- Generation of iPSC-derived Human Brain Organoids to Model Early Neurodevelopmental Disorders.Journal of visualized experiments : JoVE · 2017Article
- Induced pluripotent stem cells and Parkinson's disease: modelling and treatment.Cell proliferation · 2016Review
- Pluripotent Human embryonic stem cell derived neural lineages for in vitro modelling of enterovirus 71 infection and therapy.Virology journal · 2016Article
- Sequential Differentiation of Embryonic Stem Cells into Neural Epithelial-Like Stem Cells and Oligodendrocyte Progenitor Cells.PloS one · 2016Article
- Elevated α-synuclein caused by SNCA gene triplication impairs neuronal differentiation and maturation in Parkinson's patient-derived induced pluripotent stem cells.Cell death & disease · 2015Article
- Importance of being Nernst: Synaptic activity and functional relevance in stem cell-derived neurons.World journal of stem cells · 2015Review
- Discovery of a Novel Polymer for Human Pluripotent Stem Cell Expansion and Multilineage Differentiation.Advanced materials (Deerfield Beach, Fla.) · 2015Article
- Induced pluripotent stem cells and their use in cardiac and neural regenerative medicine.International journal of molecular sciences · 2015Review
Corrections and comments
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Efficient in vitro differentiation into specific cell types is more important than ever after the breakthrough in nuclear reprogramming of somatic cells and its potential for disease modeling and drug screening. Key success factors for neuronal differentiation are the yield of desired neuronal marker expression, reproducibility, length, and cost. Three main neuronal differentiation approaches are stromal-induced neuronal differentiation, embryoid body (EB) differentiation, and direct neuronal differentiation. Here, we describe our neurodifferentiation protocol using small molecules that very efficiently promote neural induction in a 5-stage EB protocol from six induced pluripotent stem cells (iPSC) lines from patients with Parkinson's disease and controls. This protocol generates neural precursors using Dorsomorphin and SB431542 and further maturation into dopaminergic neurons by replacing sonic hedgehog with purmorphamine or smoothened agonist. The advantage of this approach is that all patient-specific iPSC lines tested in this study were successfully and consistently coaxed into the neural lineage.
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.