ArticleFrontiers in immunology2011
Frustrated phagocytosis on micro-patterned immune complexes to characterize lysosome movements in live macrophages.
Article in Frontiers in immunology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 47 citations in OpenAlex.
- Involvement of lysosomal proteins in morphology-driven toxicity of (nano)fibers.Archives of toxicology · 2026Review
- A biophysical model of phagocytic cup dynamics: The effect of membrane tension.PLoS computational biology · 2026Article
- Polystyrene microplastic-induced pathophysiology is driven by disruption of efferocytosis.Immunity · 2026Article
- Challenges and Pitfalls of Research Designs Involving Magnesium-Based Biomaterials: An Overview.International journal of molecular sciences · 2024Review
- Spatial models of pattern formation during phagocytosis.PLoS computational biology · 2022Article
- Actin nano-architecture of phagocytic podosomes.Nature communications · 2022Article
- Cell Chirality as a Novel Measure for Cytotoxicity.Advanced biology · 2022Review
- A Lipidomics Approach to Measure Phosphatidic Acid Species in Subcellular Membrane Fractions Obtained from Cultured Cells.Bio-protocol · 2021Article
- Cellular backpacks for macrophage immunotherapy.Science advances · 2020Article
- Digestive exophagy: Phagocyte digestion of objects too large for phagocytosis.Traffic (Copenhagen, Denmark) · 2020Review
- Membrane-cytoskeletal crosstalk mediated by myosin-I regulates adhesion turnover during phagocytosis.Nature communications · 2019Article
- Biomaterials: Foreign Bodies or Tuners for the Immune Response?International journal of molecular sciences · 2019Review
- Investigation of the molecular mechanisms of hepatic injury upon naphthalene exposure in zebrafish (Danio rerio).Ecotoxicology (London, England) · 2018Article
- Mast Cells Respond toFrontiers in immunology · 2018Article
- Inhaled sildenafil as an alternative to oral sildenafil in the treatment of pulmonary arterial hypertension (PAH).Journal of controlled release : official journal of the Controlled Release Society · 2017Article
- Exocytosis of macrophage lysosomes leads to digestion of apoptotic adipocytes and foam cell formation.Journal of lipid research · 2016Article
- siFLIM: single-image frequency-domain FLIM provides fast and photon-efficient lifetime data.Nature methods · 2016Article
- Degradation of aggregated LDL occurs in complex extracellular sub-compartments of the lysosomal synapse.Journal of cell science · 2016Article
- Innate Immunity and Biomaterials at the Nexus: Friends or Foes.BioMed research international · 2015Review
- Emerging significance of NLRs in inflammatory bowel disease.Inflammatory bowel diseases · 2014Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Lysosome mobilization is a key cellular process in phagocytes for bactericidal activities and trans-matrix migration. The molecular mechanisms that regulate lysosome mobilization are still poorly known. Lysosomes are hard to track as they move toward phagosomes throughout the cell volume. In order to anticipate cell regions where lysosomes are recruited to, human and RAW264.7 macrophages were seeded on surfaces that were micro-patterned with immune complexes (ICs) as 4 μm-side squares. Distances between IC patterns were adapted to optimize cell spreading in order to constrain lysosome movements mostly in two dimensions. FcΓ receptors triggered local frustrated phagocytosis, frustrated phagosomes appeared as rings of F-actin dots around the IC patterns as early as 5 min after cells made contact with the substratum. Frustrated phagosomes recruited actin-associated proteins (vinculin, paxillin, and gelsolin). The fusion of lysosomes with frustrated phagosomes was shown by the release of beta-hexosaminidase and the recruitment of Lamp1 to frustrated phagosomes. Lysosomes of RAW264.7 macrophages were labeled with cathepsin-D-mCherry to visualize their movements toward frustrated phagosomes. Lysosomes saltatory movements were markedly slowed down compared to cells layered on non-opsonized patterns. In addition, the linearity of the trajectories and the frequency and duration of contacts of lysosomes with frustrated phagosomes were measured. Our experimental set-up is the first step toward deciphering molecular mechanisms which are involved in lysosome movements in the cytoplasm (speed, directionality, and interaction with phagosomes), and opens the door to approaches such as RNA interference, pharmacological inhibition, or mutant expression.
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Registered trials
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