ArticleMolecular and cellular biology2012
Gbx2 directly restricts Otx2 expression to forebrain and midbrain, competing with class III POU factors.
Article in Molecular and cellular biology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed.
- Combining in vitro and in silico approaches to model neural tube patterning and isthmic organizer formation.NPJ systems biology and applications · 2026Article
- Enhanced production of mesencephalic dopaminergic neurons from lineage-restricted human undifferentiated stem cells.Nature communications · 2023Article
- Clinical and Molecular Aspects Associated with Defects in the Transcription Factor POU3F4: A Review.Biomedicines · 2023Review
- Cell fate decisions, transcription factors and signaling during early retinal development.Progress in retinal and eye research · 2022Review
- An Update on the Molecular Mechanism of the Vertebrate Isthmic Organizer Development in the Context of the Neuromeric Model.Frontiers in neuroanatomy · 2022Review
- Genetic Regulation of Vertebrate Forebrain Development by Homeobox Genes.Frontiers in neuroscience · 2022Review
- Article
- Congenital pituitary hypoplasia model demonstrates hypothalamic OTX2 regulation of pituitary progenitor cells.The Journal of clinical investigation · 2020Article
- GLIS3 Transcriptionally Activates WNT Genes to Promote Differentiation of Human Embryonic Stem Cells into Posterior Neural Progenitors.Stem cells (Dayton, Ohio) · 2019Article
- Neurophilic Descending Migration of Dorsal Midbrain Neurons Into the Hindbrain.Frontiers in neuroanatomy · 2018Article
- Discovering sparse transcription factor codes for cell states and state transitions during development.eLife · 2017Article
- Midbrain-Hindbrain Boundary Morphogenesis: At the Intersection of Wnt and Fgf Signaling.Frontiers in neuroanatomy · 2017Review
- Reconstructed cell fate-regulatory programs in stem cells reveal hierarchies and key factors of neurogenesis.Genome research · 2016Article
- Engrailed 1 shapes the dopaminergic and serotonergic landscape through proper isthmic organizer maintenance and function.Biology open · 2016Article
- Unpredictable neonatal stress enhances adult anxiety and alters amygdala gene expression related to serotonin and GABA.Neuroscience · 2014Article
- Globularity and language-readiness: generating new predictions by expanding the set of genes of interest.Frontiers in psychology · 2014Article
- Chromatin accessibility mapping identifies mediators of basal transcription and retinoid-induced repression of OTX2 in medulloblastoma.PloS one · 2014Article
- Article
- Oct transcription factors in development and stem cells: insights and mechanisms.Development (Cambridge, England) · 2013Review
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4 authors.
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No grant is acknowledged in the PubMed record.
Abstract
Otx2 plays essential roles in rostral brain development, and its counteraction with Gbx2 has been suggested to determine the midbrain-hindbrain boundary (MHB) in vertebrates. We previously identified the FM enhancer that is conserved among vertebrates and drives Otx2 transcription in forebrain/midbrain from the early somite stage. In this study, we found that the POU homeodomain of class III POU factors (Brn1, Brn2, Brn4, and Oct6) associates with noncanonical target sequence TAATTA in the FM enhancer. MicroRNA-mediated knockdown of Brn2 and Oct6 diminished the FM enhancer activity in anterior neural progenitor cells (NPCs) differentiated from P19 cells. The class III POU factors associate with the FM enhancer in forebrain and midbrain but not in hindbrain. We also demonstrated that the Gbx2 homeodomain recognizes the same target TAATTA in the FM enhancer, and Gbx2 associates with the FM enhancer in hindbrain. Gbx2 misexpression in the anterior NPCs repressed the FM enhancer activity and inhibited Brn2 association with the enhancer, whereas Gbx2 knockdown caused ectopic Brn2 association in the posterior NPCs. These results suggest that class III POU factors and Gbx2 share the same target site, TAATTA, in the FM enhancer and that their region-specific binding restricts Otx2 expression at the MHB.
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