ArticleProceedings of the National Academy of Sciences of the United States of America2012
Gene 5.5 protein of bacteriophage T7 in complex with Escherichia coli nucleoid protein H-NS and transfer RNA masks transfer RNA priming in T7 DNA replication.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed.
- tRNA-derived small RNAs in disease immunity.Theranostics · 2025Review
- tRNA and tsRNA: From Heterogeneity to Multifaceted Regulators.Biomolecules · 2024Review
- AInternational journal of molecular sciences · 2023Article
- Novel anti-repression mechanism of H-NS proteins by a phage protein.Nucleic acids research · 2021Article
- Noncanonical Roles of tRNAs: tRNA Fragments and Beyond.Annual review of genetics · 2020Review
- Full shut-off of Escherichia coli RNA-polymerase by T7 phage requires a small phage-encoded DNA-binding protein.Nucleic acids research · 2017Article
- A genetically encoded fluorescent tRNA is active in live-cell protein synthesis.Nucleic acids research · 2017Article
- Characterization of Five Podoviridae Phages Infecting Citrobacter freundii.Frontiers in microbiology · 2016Article
- Antibacterial phage ORFans of Pseudomonas aeruginosa phage LUZ24 reveal a novel MvaT inhibiting protein.Frontiers in microbiology · 2015Article
- Single-molecule studies on the mechanical interplay between DNA supercoiling and H-NS DNA architectural properties.Nucleic acids research · 2014Article
- Syn5 RNA polymerase synthesizes precise run-off RNA products.Nucleic acids research · 2014Article
- Flap endonuclease activity of gene 6 exonuclease of bacteriophage T7.The Journal of biological chemistry · 2014Article
- H-NST induces LEE expression and the formation of attaching and effacing lesions in enterohemorrhagic Escherichia coli.PloS one · 2014Article
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5 authors.
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Abstract
DNA primases provide oligoribonucleotides for DNA polymerase to initiate lagging strand synthesis. A deficiency in the primase of bacteriophage T7 to synthesize primers can be overcome by genetic alterations that decrease the expression of T7 gene 5.5, suggesting an alternative mechanism to prime DNA synthesis. The product of gene 5.5 (gp5.5) forms a stable complex with the Escherichia coli histone-like protein H-NS and transfer RNAs (tRNAs). The 3'-terminal sequence (5'-ACCA-3') of tRNAs is identical to that of a functional primer synthesized by T7 primase. Mutations in T7 that suppress the inability of primase reduce the amount of gp5.5 and thus increase the pool of tRNA to serve as primers. Alterations in T7 gene 3 facilitate tRNA priming by reducing its endonuclease activity that cleaves at the tRNA-DNA junction. The tRNA bound to gp5.5 recruits H-NS. H-NS alone inhibits reactions involved in DNA replication, but the binding to gp5.5-tRNA complex abolishes this inhibition.
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