ArticleFish physiology and biochemistry2012
The effect of fasting and refeeding on mRNA expression of PepT1 and gastrointestinal hormones regulating digestion and food intake in zebrafish (Danio rerio).
Article in Fish physiology and biochemistry, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 62 citations in OpenAlex.
- Impact of maternal DHA-enriched plant-based diets on early development of rainbow trout offspring: insights into parental nutritional programming.Marine life science & technology · 2025Article
- Acute Stress Effects over Time on Gene Expression Patterns in the Male Zebrafish (Animals : an open access journal from MDPI · 2025Article
- Chronic social isolation affects feeding behavior of juvenile zebrafish (Danio rerio).PloS one · 2024Article
- Effect of Early Peptide Diets on Zebrafish Skeletal Development.Biomolecules · 2023Article
- Effects of different acute stressors on the regulation of appetite genes in the carp (Royal Society open science · 2023Article
- The Interplay between Central and Peripheral Systems in Feed Intake Regulation in European Seabass (Animals : an open access journal from MDPI · 2022Article
- An Integrated Bioinformatics Approach to Identify Network-Derived Hub Genes in Starving Zebrafish.Animals : an open access journal from MDPI · 2022Article
- Combined blue light and daily thermocycles enhance zebrafish growth and development.Journal of experimental zoology. Part A, Ecological and integrative physiology · 2022Article
- Article
- Effects of Short-Term Fasting on mRNA Expression of Ghrelin and the Peptide Transporters PepT1 and 2 in Atlantic Salmon (Frontiers in physiology · 2021Article
- Growth Hormone Overexpression Induces Hyperphagia and Intestinal Morphophysiological Adaptations to Improve Nutrient Uptake in Zebrafish.Frontiers in physiology · 2021Article
- Growth hormone regulates intestinal gene expression of nutrient transporters in tilapia (Oreochromis mossambicus).General and comparative endocrinology · 2020Article
- Nutritional programming improves dietary plant protein utilization in zebrafish Danio rerio.PloS one · 2020Article
- Nutrient Regulation of Endocrine Factors Influencing Feeding and Growth in Fish.Frontiers in endocrinology · 2019Review
- The peptide transporter 1a of the zebrafishGenes & nutrition · 2019Article
- Peptide Transporters in the Primary Gastrointestinal Tract of Pre-Feeding Mozambique Tilapia Larva.Frontiers in physiology · 2019Article
- Spatial mRNA Expression and Response to Fasting and Refeeding of Neutral Amino Acid TransportersFrontiers in physiology · 2018Article
- Fasting UpregulatesFrontiers in physiology · 2018Article
- Immunohistochemical Characterization of PepT1 and Ghrelin in Gastrointestinal Tract of Zebrafish: Effects ofFrontiers in physiology · 2018Article
- Di- and tripeptide transport in vertebrates: the contribution of teleost fish models.Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology · 2017Review
Corrections and comments
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Authors and funding
2 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In vertebrates, a significant part of ingested protein is absorbed as di- and tripeptides through a brush border membrane proton/oligopeptide transporter protein called PepT1. The aim of the present study was to determine the effect of short-term food deprivation and refeeding in adult zebrafish (Danio rerio) on gastrointestinal mRNA expression of PepT1 as well as on the satiety hormones cholecystokinin (CCK), gastrin-releasing peptide (GRP) and ghrelin (GHR) in order to elucidate a potential mechanism driving compensatory growth. Sixty adult zebrafish were stocked in a 40-L aquarium and fed daily a commercial flake diet to satiation for 10 days where the digestive tracts (DT) of sampled fish (n = 5) were dissected out. Samplings were repeated following 1, 2 and 5 days of food deprivation and after 1, 2 and 5 days of refeeding. The RNA was extracted from all sampled DTs and analyzed by quantitative real-time PCR for the mRNA expression of PepT1, rRNA 18S, CCK, GRP and GHR. PepT1 mRNA expression increased with successive refeedings reaching a level approximately 8 times higher than pre-fast levels. CCK, GRP and GHR mRNA levels also decreased during fasting, but increased only to pre-fasting levels with refeeding. Overall, the results suggest that PepT1 may be a contributing mechanism to compensatory growth that could influence CCK secretion and GRP and GHR activity.
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