ArticleMolecules (Basel, Switzerland)2012
Lectin from Canavalia brasiliensis seeds (ConBr) is a valuable biotechnological tool to stimulate the growth of Rhizobium tropici in vitro.
Article in Molecules (Basel, Switzerland), 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
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4 citing papers in PubMed, 13 citations in OpenAlex.
- Preparation ofBiomedical reports · 2018Article
- Effect of algae and plant lectins on planktonic growth and biofilm formation in clinically relevant bacteria and yeasts.BioMed research international · 2014Article
- Effect of leguminous lectins on the growth of Rhizobium tropici CIAT899.Molecules (Basel, Switzerland) · 2013Article
- Toxicity and binding profile of lectins from the Genus canavalia on brine shrimp.BioMed research international · 2013Article
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11 authors at 2 institutions in 1 country.
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Abstract
To study the interactions between a Rhizobium tropici strain and lectins isolated from the seeds of Canavalia ensiformis (ConA) and Canavalia brasiliensis (ConBr), a lectin fluorescence assay was performed. In addition, an experiment was designed to evaluate the effect of the two lectins on bacterial growth. Both lectins were found to bind to R. tropici cells, but the interactions were inhibited by D-mannose. Interestingly, only ConBr stimulated bacterial growth in proportion to the concentrations used (15.6-500 μg/mL), and the bacterial growth stimulation was inhibited by D-mannose as well. Structure/Function analyses by bioinformatics were carried out to evaluate the volume and carbohydrate recognition domain (CRD) configuration of ConA and ConBr. The difference of spatial arrangement and volume of CRD may indicate the variation between biological activities of both lectins. The results suggest that ConBr could be a promising tool for studies focusing on the interactions between rhizobia and host plants.
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