ArticleThe Journal of surgical research2012
Intestinal crypts reproducibly expand in culture.
Article in The Journal of surgical research, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.
What it found
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
42 citing papers in PubMed, 65 citations in OpenAlex.
- Intestinal organoid screen reveals that Bacillus velezensis PGM541 promotes epithelial proliferation via its metabolite butyric acid.Microbiome · 2026Article
- Integrated Stress Response and Necroptosis Drive Epithelial Dysfunction in Crohn's Disease: Repurposing Cancer Drugs for Permeability Barrier Healing.Gastro hep advances · 2026Article
- Development of Sheep Intestinal Organoids for Studying Deoxynivalenol-Induced Toxicity.International journal of molecular sciences · 2025Article
- In Vitro Culture of Human Norovirus in the Last 20 Years.Biomedicines · 2024Review
- Current trends and new approaches for human norovirus replication in cell culture: a literature review.Archives of virology · 2024Review
- Effects of β-alanine on intestinal development and immune performance of weaned piglets.Animal nutrition (Zhongguo xu mu shou yi xue hui) · 2023Article
- Culture of equine intestinal epithelial stem cells after delayed tissue storage for future applications.BMC veterinary research · 2022Article
- Paneth Cells Protect against Acute Pancreatitis via Modulating Gut Microbiota Dysbiosis.mSystems · 2022Article
- Dietary High Dose of Iron Aggravates the Intestinal Injury but Promotes Intestinal Regeneration by Regulating Intestinal Stem Cells Activity in Adult Mice With Dextran Sodium Sulfate-Induced Colitis.Frontiers in veterinary science · 2022Article
- HOPXAmerican journal of physiology. Gastrointestinal and liver physiology · 2021Article
- Dietary Copper Improves Intestinal Morphology via Modulating Intestinal Stem Cell Activity in Pigs.Animals : an open access journal from MDPI · 2021Article
- Review
- Gastrointestinal organoids: a next-generation tool for modeling human development.American journal of physiology. Gastrointestinal and liver physiology · 2020Review
- Regulation of Enteroendocrine Cell Networks by the Major Human Gut SymbiontFrontiers in microbiology · 2020Article
- NF-κB2 signalling in enteroids modulates enterocyte responses to secreted factors from bone marrow-derived dendritic cells.Cell death & disease · 2019Article
- Developing a 3D intestinal epithelium model for livestock species.Cell and tissue research · 2019Article
- The enteric microbiota regulates jejunal Paneth cell number and function without impacting intestinal stem cells.Gut microbes · 2019Article
- CRISPR therapeutic tools for complex genetic disorders and cancer (Review).International journal of oncology · 2018Review
- The LIN28B-IMP1 post-transcriptional regulon has opposing effects on oncogenic signaling in the intestine.Genes & development · 2018Article
- Epimorphin regulates the intestinal stem cell niche via effects on the stromal microenvironment.American journal of physiology. Gastrointestinal and liver physiology · 2018Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
backgroundIn vitro growth techniques for intestinal crypts and single intestinal stem cells have been recently described, but several questions of translational importance remain unaddressed. The purpose of this study was to first, evaluate if intestinal crypts reproducibly expand in vitro; second, determine the impact of age and region of intestine on crypt growth in vitro; and third, determine the effects of cryopreservation on crypt growth in vitro. METHODS AND MATERIALS: Crypts were harvested from 5 cm of proximal, middle, and distal small intestine of C57BL/6J mice aged 4 wk, 6-8 wk, 12-14 wk, and 18-20 wk (n = 4-6 animals) and cultured. For each region, we determined the efficiency of crypts forming enterospheres (day 1) and progressing to enteroids (day 7). Subsequently, enteroids were passaged and cryopreserved to determine if growth was changed by these manipulations.
resultsForty-three to 99% of intestinal crypts formed enterospheres, with higher efficiency in proximal small intestine and in younger mice. Twenty-five to 64% of enterospheres progressed to budding enteroids within 7 d. In vitro expansion was greater in proximal enteroids. This expansion continued in a logarithmic fashion, with ≈ 97% replating efficiency of isolated enteroid crypt buds. Following cryopreservation, ≈ 90% of enteroids recovered normal proliferative capacity.
conclusionsIntestinal crypt culture is efficient and significantly expands intestinal tissue in a reproducible manner. Regional and age growth differences may reflect distinct stem cell characteristics or differences in support cells. The ability to culture and expand intestinal tissue in vitro provides a potential translational approach toward understanding and treating patients with short bowel syndrome.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.