ArticleWound repair and regeneration : official publication of the Wound Healing Society [and] the European Tissue Repair Society
Nonviral human beta defensin-3 expression in a bioengineered human skin tissue: a therapeutic alternative for infected wounds.
Article in Wound repair and regeneration : official publication of the Wound Healing Society [and] the European Tissue Repair Society. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 34 citations in OpenAlex.
- A Systematic Review of the Design and Applications of Antimicrobial Peptides in Wound Healing.Cureus · 2024Review
- Living Skin Substitute Tissue-Is a Replacement for the Autograft Possible?European burn journal · 2023Article
- Mammals' humoral immune proteins and peptides targeting the bacterial envelope: from natural protection to therapeutic applications against multidrug-resistant Gram-negatives.Biological reviews of the Cambridge Philosophical Society · 2022Review
- Non-viral gene delivery systems for tissue repair and regeneration.Journal of translational medicine · 2018Review
- Tissue engineering of skin and regenerative medicine for wound care.Burns & trauma · 2018Review
- Progress and Future Prospectives in Skin-on-Chip Development with Emphasis on the use of Different Cell Types and Technical Challenges.Stem cell reviews and reports · 2017Review
- New Is Old, and Old Is New: Recent Advances in Antibiotic-Based, Antibiotic-Free and Ethnomedical Treatments against Methicillin-Resistant Staphylococcus aureus Wound Infections.International journal of molecular sciences · 2016Review
- Development of a Full-Thickness Human Skin Equivalent In Vitro Model Derived from TERT-Immortalized Keratinocytes and Fibroblasts.Tissue engineering. Part A · 2015Article
- [Not Available].Annals of burns and fire disasters · 2015Article
- Article
- Human beta-defensin 3: a novel inhibitor of Staphylococcus-produced biofilm production. Commentary on "Human β-defensin 3 inhibits antibiotic-resistant Staphylococcus biofilm formation".The Journal of surgical research · 2014Article
- [The skin's own antibiotics. Important features of antimicrobial peptides for clinical practice].Der Hautarzt; Zeitschrift fur Dermatologie, Venerologie, und verwandte Gebiete · 2014Article
- Immune modulation by multifaceted cationic host defense (antimicrobial) peptides.Nature chemical biology · 2013Review
- Topical antimicrobials for burn infections - an update.Recent patents on anti-infective drug discovery · 2013Review
- Ultrasound-targeted microbubble destruction enhances human β-defensin 3 activity against antibiotic-resistant Staphylococcus biofilms.Inflammation · 2013Article
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Authors and funding
10 authors at 4 institutions in 2 countries.
Funding
Abstract
The innate immune system differentially regulates the expression of host defense peptides to combat infection during wound healing. We enhanced the expression of a host defense peptide, human beta defensin-3 (hBD-3), in keratinocytes to generate a three-dimensional biologic dressing to improve healing of infected wounds. The NIKS human keratinocyte cell line was stably transfected ex vivo with a construct containing an epidermis-specific promoter driving hBD-3 (NIKS(hBD) (-3) ) using nonviral methods. Levels of hBD-3 mRNA and protein in three-dimensional skin tissue produced from NIKS(hBD) (-3) were determined using quantitative polymerase chain reaction and enzyme-linked immunosorbent assay, respectively. Tissue architecture was characterized by hematoxylin and eosin staining and by indirect immunofluorescence using proliferation and keratinocyte differentiation markers. Antimicrobial activity was assessed using an in vitro bacterial growth assay and in vivo using a murine burn infection model. Three-dimensional full thickness skin tissues containing epidermal NIKS(hBD) (-3) or control NIKS possessed histologic features of interfollicular epidermis and exhibited normal tissue growth and differentiation. NIKS(hBD) (-3) tissue contained approximately fivefold more hBD-3 protein than tissue containing unmodified control NIKS. In vitro studies showed that NIKS(hBD) (-3) tissue produced a significant reduction in the growth of Staphylococcus aureus multiple peptide resistance factor (mprF) compared with control tissue. In an in vivo infected murine burn model, NIKS(hBD) (-3) tissue resulted in a 90% reduction in bacterial growth. These results demonstrate that sustained delivery of hBD-3 by a bioengineered skin tissue results in a therapeutically relevant reduction in growth of a S. aureus strain in an animal model of infected third-degree burn wounds.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.