ArticleWorld journal of gastroenterology2012
Probiotic metabolites from Bacillus coagulans GanedenBC30™ support maturation of antigen-presenting cells in vitro.
Article in World journal of gastroenterology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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The trial behind it
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Who cites it
20 citing papers in PubMed, 41 citations in OpenAlex.
- Trial
- Bacillus coagulans BC66 Attenuates Klebsiella pneumoniae-Induced Acute Lung Injury in Rabbits Associated With Modulation of Th17/Treg Immune Balance and Through the Keap1/Nrf2/HO-1 Signalling Pathway.Probiotics and antimicrobial proteins · 2026Article
- Germinated Spores of the Probiotic BacteriumMicroorganisms · 2025Article
- Winning the battle of intestinal peace withFrontiers in microbiology · 2025Review
- Harnessing the dual nature of Bacillus (Weizmannia) coagulans for sustainable production of biomaterials and development of functional food.Microbial biotechnology · 2024Review
- Differential Immune-Modulating Activities of Cell Walls and Secreted Metabolites from ProbioticMicroorganisms · 2023Article
- Microbiome and Probiotics in Acne Vulgaris-A Narrative Review.Life (Basel, Switzerland) · 2022Review
- Modulation of Gut Microbiota and Immune System by Probiotics, Pre-biotics, and Post-biotics.Frontiers in nutrition · 2021Review
- Application ofAnimals : an open access journal from MDPI · 2020Review
- Potential Role of the Microbiome in Acne: A Comprehensive Review.Journal of clinical medicine · 2019Review
- Potential Use ofFoods (Basel, Switzerland) · 2018Review
- Implications of Probiotics on the Maternal-Neonatal Interface: Gut Microbiota, Immunomodulation, and Autoimmunity.Frontiers in immunology · 2018Review
- Diet and rosacea: the role of dietary change in the management of rosacea.Dermatology practical & conceptual · 2017Review
- Inhibition of Lipopolysaccharide-Induced Interleukin 8 in Human Adenocarcinoma Cell Line HT-29 by Spore Probiotics: B. coagulans and B. subtilis (natto).Probiotics and antimicrobial proteins · 2017Article
- Inactivated probioticJournal of inflammation research · 2017Article
- The effect of probiotics on immune regulation, acne, and photoaging.International journal of women's dermatology · 2015Review
- Anti-inflammatory properties of a dried fermentate in vitro and in vivo.Journal of medicinal food · 2015Article
- Microorganisms with claimed probiotic properties: an overview of recent literature.International journal of environmental research and public health · 2014Review
- Recovery of Viable Bacteria from Probiotic Products that Target Oral Health.Probiotics and antimicrobial proteins · 2013Article
- Oral microbial shift induced by probiotic Bacillus coagualans along with its clinical perspectives.Journal of oral biology and craniofacial researchReview
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
aimTo study the effects of probiotic metabolites on maturation stage of antigen-presenting immune cells.
methodsGaneden Bacillus coagulans 30 (GBC30) bacterial cultures in log phase were used to isolate the secreted metabolite (MET) fraction. A second fraction was made to generate a crude cell-wall-enriched fraction, by centrifugation and lysis, followed by washing. A preparation of MET was subjected to size exclusion centrifugation, generating three fractions: < 3 kDa, 3-30 kDa, and 30-200 kDa and activities were tested in comparison to crude MET and cell wall in primary cultures of human peripheral blood mononuclear cell (PBMC) as a source of antigen-presenting mononuclear phagocytes. The maturation status of mononuclear phagocytes was evaluated by staining with monoclonal antibodies towards CD14, CD16, CD80 and CD86 and analyzed by flow cytometry.
resultsTreatment of PBMC with MET supported maturation of mononuclear phagocytes toward both macrophage and dendritic cell phenotypes. The biological activity unique to the metabolites included a reduction of CD14+ CD16+ pro-inflammatory cells, and this property was associated with the high molecular weight metabolite fraction. Changes were also seen for the dendritic cell maturation markers CD80 and CD86. On CD14(dim) cells, an increase in both CD80 and CD86 expression was seen, in contrast to a selective increase in CD86 expression on CD14(bright) cells. The co-expression of CD80 and CD86 indicates effective antigen presentation to T cells and support of T helper cell differentiation. The selective expression of CD86 in the absence of CD80 points to a role in generating T regulatory cells.
conclusionThe data show that a primary mechanism of action of GBC30 metabolites involves support of more mature phenotypes of antigen-presenting cells, important for immunological decision-making.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.