ArticleGenetics2012
A hyperactive transposase of the maize transposable element activator (Ac).
Article in Genetics, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 31 citations in OpenAlex.
- Progresses of transposon research inSynthetic and systems biotechnology · 2026Review
- Landscape of essential growth and fluconazole-resistance genes in the human fungal pathogen Cryptococcus neoformans.PLoS biology · 2025Article
- Activity of the mammalian DNA transposon piggyBat from Myotis lucifugus is restricted by its own transposon ends.Nature communications · 2025Article
- Transposase expression, element abundance, element size, and DNA repair determine the mobility and heritability ofFrontiers in cell and developmental biology · 2023Article
- Distinct composition and amplification dynamics of transposable elements in sacred lotus (Nelumbo nucifera Gaertn.).The Plant journal : for cell and molecular biology · 2022Article
- Transposon insertional mutagenesis of diverse yeast strains suggests coordinated gene essentiality polymorphisms.Nature communications · 2022Article
- Artificial optimization of bambooFrontiers in plant science · 2022Article
- SAturated Transposon Analysis in Yeast (SATAY) for Deep Functional Mapping of Yeast Genomes.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Article
- Hijacking Transposable Elements for Saturation Mutagenesis in Fungi.Frontiers in fungal biology · 2021Review
- Insertion Specificity of the hATx-6 Transposase ofFrontiers in molecular biosciences · 2021Article
- Comparing the utility of in vivo transposon mutagenesis approaches in yeast species to infer gene essentiality.Current genetics · 2020Article
- Host-transposon interactions: conflict, cooperation, and cooption.Genes & development · 2019Review
- Gene Essentiality Analyzed bymBio · 2018Article
- Maize Transposable ElementsG3 (Bethesda, Md.) · 2018Article
- Article
- Vast potential for using the piggyBac transposon to engineer transgenic plants at specific genomic locations.Bioengineered · 2016Article
- A small indel mutation in an anthocyanin transporter causes variegated colouration of peach flowers.Journal of experimental botany · 2015Article
- Transposition of a rice Mutator-like element in the yeast Saccharomyces cerevisiae.The Plant cell · 2015Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Activator/Dissociation (Ac/Ds) transposable elements from maize are widely used as insertional mutagenesis and gene isolation tools in plants and more recently also in medaka and zebrafish. They are particularly valuable for plant species that are transformation-recalcitrant and have long generation cycles or large genomes with low gene densities. Ac/Ds transposition frequencies vary widely, however, and in some species they are too low for large-scale mutagenesis. We discovered a hyperactive Ac transposase derivative, AcTPase(4x), that catalyzes in the yeast Saccharomyces cerevisiae 100-fold more frequent Ds excisions than the wild-type transposase, whereas the reintegration frequency of excised Ds elements is unchanged (57%). Comparable to the wild-type transposase in plants, AcTPase(4x) catalyzes Ds insertion preferentially into coding regions and to genetically linked sites, but the mutant protein apparently has lost the weak bias of the wild-type protein for insertion sites with elevated guanine-cytosine content and nonrandom protein-DNA twist. AcTPase(4x) exhibits hyperactivity also in Arabidopsis thaliana where it effects a more than sixfold increase in Ds excision relative to wild-type AcTPase and thus may be useful to facilitate Ac/Ds-based insertion mutagenesis approaches.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.