ArticleProceedings of the National Academy of Sciences of the United States of America2012
Allosteric inhibition of individual enzyme molecules trapped in lipid vesicles.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
25 citing papers in PubMed, 76 citations in OpenAlex.
- Fluorogenic Reactions in Chemical Biology: Seeing Chemistry in Cells.Chemical & biomedical imaging · 2023Review
- Dynamic Heterogeneity in the Optical Signals from Single Nano-Objects.The journal of physical chemistry. B · 2023Review
- Single-Molecule FRET of Membrane Transport Proteins.Chembiochem : a European journal of chemical biology · 2021Review
- Advances in Optical Single-Molecule Detection: En Route to Supersensitive Bioaffinity Assays.Angewandte Chemie (International ed. in English) · 2020Review
- Bioinspired Molecular Factories with Architecture and In Vivo Functionalities as Cell Mimics.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2020Article
- Quantitative analysis of HMethodsX · 2020Article
- Single electron transfer events and dynamical heterogeneity in the small protein azurin fromChemical science · 2019Article
- Guiding protein delivery into live cells using DNA-programmed membrane fusion.Chemical science · 2018Article
- Cell Membrane-Based Nanoreactor To Mimic the Bio-Compartmentalization Strategy of a Cell.ACS biomaterials science & engineering · 2018Article
- Single-molecule theory of enzymatic inhibition.Nature communications · 2018Article
- Slow domain reconfiguration causes power-law kinetics in a two-state enzyme.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Decoding Single Molecule Time Traces with Dynamic Disorder.PLoS computational biology · 2016Article
- Protein Ensembles: How Does Nature Harness Thermodynamic Fluctuations for Life? The Diverse Functional Roles of Conformational Ensembles in the Cell.Chemical reviews · 2016Review
- Enzymatic reactions in confined environments.Nature nanotechnology · 2016Review
- Principles of allosteric interactions in cell signaling.Journal of the American Chemical Society · 2014Review
- Shedding light on protein folding, structural and functional dynamics by single molecule studies.Molecules (Basel, Switzerland) · 2014Review
- Enzyme molecules in solitary confinement.Molecules (Basel, Switzerland) · 2014Review
- The free energy landscape in translational science: how can somatic mutations result in constitutive oncogenic activation?Physical chemistry chemical physics : PCCP · 2014Article
- Rate of solute incorporation to liposomes evaluated from encapsulated enzymes activities.Biophysical reviews · 2014Review
- Antibiotic activity against naive and induced Streptococcus pneumoniae biofilms in an in vitro pharmacodynamic model.Antimicrobial agents and chemotherapy · 2014Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 2 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Enzymatic inhibition by product molecules is an important and widespread phenomenon. We describe an approach to study product inhibition at the single-molecule level. Individual HRP molecules are trapped within surface-tethered lipid vesicles, and their reaction with a fluorogenic substrate is probed. While the substrate readily penetrates into the vesicles, the charged product (resorufin) gets trapped and accumulates inside the vesicles. Surprisingly, individual enzyme molecules are found to stall when a few tens of product molecules accumulate. Bulk enzymology experiments verify that the enzyme is noncompetitively inhibited by resorufin. The initial reaction velocity of individual enzyme molecules and the number of product molecules required for their complete inhibition are broadly distributed and dynamically disordered. The two seemingly unrelated parameters, however, are found to be substantially correlated with each other in each enzyme molecule and over long times. These results suggest that, as a way to counter disorder, enzymes have evolved the means to correlate fluctuations at structurally distinct functional sites.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.