ArticlePlant molecular biology2012
Polyuridylylation and processing of transcripts from multiple gene minicircles in chloroplasts of the dinoflagellate Amphidinium carterae.
Article in Plant molecular biology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 26 citations in OpenAlex.
- Interpreting the complexities of the plastid genome in dinoflagellates: a mini-review of recent advances.Plant molecular biology · 2024Review
- Mini-synplastomes for plastid genetic engineering.Plant biotechnology journal · 2022Article
- Article
- Plastid Transcript Editing across Dinoflagellate Lineages Shows Lineage-Specific Application but Conserved Trends.Genome biology and evolution · 2018Article
- Pervasive, Genome-Wide Transcription in the Organelle Genomes of Diverse Plastid-Bearing Protists.G3 (Bethesda, Md.) · 2017Article
- Major transitions in dinoflagellate evolution unveiled by phylotranscriptomics.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Transcripts in the Plasmodium Apicoplast Undergo Cleavage at tRNAs and Editing, and Include Antisense Sequences.Protist · 2016Article
- Diversity of transcripts and transcript processing forms in plastids of the dinoflagellate alga Karenia mikimotoi.Plant molecular biology · 2016Article
- Integration of plastids with their hosts: Lessons learned from dinoflagellates.Proceedings of the National Academy of Sciences of the United States of America · 2015Review
- Genome-wide transcript profiling reveals the coevolution of plastid gene sequences and transcript processing pathways in the fucoxanthin dinoflagellate Karlodinium veneficum.Molecular biology and evolution · 2014Article
- Massive gene transfer and extensive RNA editing of a symbiotic dinoflagellate plastid genome.Genome biology and evolution · 2014Article
- Evolution of chloroplast transcript processing in Plasmodium and its chromerid algal relatives.PLoS genetics · 2014Article
- Functional remodeling of RNA processing in replacement chloroplasts by pathways retained from their predecessors.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- Transcription of the apicoplast genome.Molecular and biochemical parasitologyReview
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Authors and funding
6 authors at 1 institution in 2 countries.
Funding
Abstract
Although transcription and transcript processing in the chloroplasts of plants have been extensively characterised, the RNA metabolism of other chloroplast lineages across the eukaryotes remains poorly understood. In this paper, we use RT-PCR to study transcription and transcript processing in the chloroplasts of Amphidinium carterae, a model peridinin-containing dinoflagellate. These organisms have a highly unusual chloroplast genome, with genes located on multiple small 'minicircle' elements, and a number of idiosyncratic features of RNA metabolism including transcription via a rolling circle mechanism, and 3' terminal polyuridylylation of transcripts. We demonstrate that transcription occurs in A. carterae via a rolling circle mechanism, as previously shown in the dinoflagellate Heterocapsa, and present evidence for the production of both polycistronic and monocistronic transcripts from A. carterae minicircles, including several regions containing ORFs previously not known to be expressed. We demonstrate the presence of both polyuridylylated and non-polyuridylylated transcripts in A. carterae, and show that polycistronic transcripts can be terminally polyuridylylated. We present a model for RNA metabolism in dinoflagellate chloroplasts where long polycistronic precursors are processed to form mature transcripts. Terminal polyuridylylation may mark transcripts with the correct 3' end.
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