ArticleInvestigative ophthalmology & visual science2012
Downregulation of β-actin gene and human antigen R in human keratoconus.
Article in Investigative ophthalmology & visual science, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers, 1 of them a synthesis that pooled it.
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Who cites it
19 citing papers in PubMed, 1 synthesis or guideline pooled it, 28 citations in OpenAlex.
- Systematically Displaying the Pathogenesis of KeratoconusFrontiers in medicine · 2021Pooled it
- Multimodal AI/ML for discovering novel biomarkers and predicting disease using multi-omics profiles of patients with cardiovascular diseases.Scientific reports · 2024Article
- Identification of hub genes and molecular pathways in keratoconus by integrating bioinformatics and literature mining at the RNA level.International ophthalmology · 2024Article
- Upregulation of HPV16E1 and E7 expression and FOXO3a mRNA downregulation in high-grade cervical neoplasia.PeerJ · 2024Article
- Role of Fibroblast Growth Factor Receptor 2 (FGFR2) in Corneal Stromal Thinning.Investigative ophthalmology & visual science · 2023Article
- RNA-binding proteins and post-transcriptional regulation in lens biology and cataract: Mediating spatiotemporal expression of key factors that control the cell cycle, transcription, cytoskeleton and transparency.Experimental eye research · 2022Review
- Actin Polymerization Is Required for Filopodia Formation Supporting HSV-1 Entry into Activated T Cells.Current microbiology · 2021Article
- The Effect of Acute and Chronic Thermotherapy on Type 2 Diabetic Skeletal Muscle Gene Expression and Inflammatory Markers.Biomedicines · 2021Article
- Role of theMolecular vision · 2020Article
- The Proteins of Keratoconus: a Literature Review Exploring Their Contribution to the Pathophysiology of the Disease.Advances in therapy · 2019Review
- Transcriptional profiling of corneal stromal cells derived from patients with keratoconus.Scientific reports · 2019Article
- The Role of Endogenous Neuroprotective Mechanisms in the Prevention of Retinal Ganglion Cells Degeneration.Frontiers in neuroscience · 2018Review
- Molecular and Histopathological Changes Associated with Keratoconus.BioMed research international · 2017Review
- Modeling Keratoconus Using Induced Pluripotent Stem Cells.Investigative ophthalmology & visual science · 2016Article
- RNA-binding proteins in eye development and disease: implication of conserved RNA granule components.Wiley interdisciplinary reviews. RNA · 2016Review
- Tear biomarkers for keratoconus.Eye and vision (London, England) · 2016Review
- Keratoconus: an inflammatory disorder?Eye (London, England) · 2015Review
- Downregulation of β-actin and its regulatory gene HuR affect cell migration of human corneal fibroblasts.Molecular vision · 2014Article
- Abnormal regulation of extracellular matrix and adhesion molecules in corneas of patients with keratoconus.International journal of keratoconus and ectatic corneal diseasesArticle
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
purposeThe purpose of this study was to determine the expression levels and regulation of β-actin in the stroma of keratoconus (KC) and normal corneas.
methodsA total of 15 different human corneas from both KC and normal individuals were used for this study. Additionally, 3 Fuch's dystrophic corneas were also used. The β-actin gene expression was analyzed at the transcriptional and translational levels in the epithelium and stroma of the KC and normal corneas. The human antigen R (HuR) gene expression was analyzed by real-time PCR in the stroma of five KC and five normal corneas. The keratocytes from three normal and three KC corneas were cultured in the presence of serum, and the expression levels of β-actin and human antigen R (HuR) were analyzed by using confocal imaging in both normal and KC fibroblasts.
resultsThe expression of the β-actin gene was downregulated in the stroma of the six KC corneas but not in the stroma of six normal and Fuchs' dystrophic corneas. Immunofluorescence detection of β-actin showed that it was absent in the KC fibroblast. The real-time PCR analysis of the HuR gene showed a relative 4.7-fold lower expression in KC corneas relative to the normal corneas, which was further confirmed by the immunofluorescence detection of HuR in fibroblasts of KC corneas.
conclusionsAlthough ubiquitous β-actins are essential for cell survival during early embryogenesis, the effects on various stages of development are not well understood. Our results show that β-actin is downregulated in the corneal stroma of patients with KC, which may be related to reduced levels of a stabilizing factor (HuR) for β-actin mRNA. We propose that loss of β-actin in the corneal stroma might be a triggering factor in the development of KC.
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