ArticleMarine biotechnology (New York, N.Y.)2013
Genomic mining for novel FADH₂-dependent halogenases in marine sponge-associated microbial consortia.
Article in Marine biotechnology (New York, N.Y.), 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed.
- Substrate-driven microbial specialization and cooperative dechlorination of chlorinated pollutants in estuarine ecosystems.Applied and environmental microbiology · 2026Article
- Precision Molecular Editing: Predicting Substrate Scope and Regiochemistry for CHEESY1, a Flavin Dependent Halogenase.ACS catalysis · 2025Article
- Structural basis of regioselective tryptophan dibromination by the single-component flavin-dependent halogenase AetF.Acta crystallographica. Section D, Structural biology · 2023Article
- Organohalide respiration potential in marine sediments from Aarhus Bay.FEMS microbiology ecology · 2022Article
- Community composition and functional prediction of prokaryotes associated with sympatric sponge species of southwestern Atlantic coast.Scientific reports · 2021Article
- Harnessing the sponge microbiome for industrial biocatalysts.Applied microbiology and biotechnology · 2020Review
- Organohalide-respiring Desulfoluna species isolated from marine environments.The ISME journal · 2020Article
- Cultivation of Bacteria FromFrontiers in microbiology · 2020Article
- Halogenating Enzymes for Active Agent Synthesis: First Steps Are Done and Many Have to Follow.Molecules (Basel, Switzerland) · 2019Review
- Diversity of tryptophan halogenases in sponges of the genus Aplysina.FEMS microbiology ecology · 2019Article
- Bioactivities of Halometabolites from Marine Actinobacteria.Biomolecules · 2019Review
- Microbial and Functional Biodiversity Patterns in Sponges that Accumulate Bromopyrrole Alkaloids Suggest Horizontal Gene Transfer of Halogenase Genes.Microbial ecology · 2018Article
- Microbiomes of the Enteropneust,Frontiers in microbiology · 2018Article
- Microbial Synthesis and Transformation of Inorganic and Organic Chlorine Compounds.Frontiers in microbiology · 2018Review
- Enzymatic Halogenation and Dehalogenation Reactions: Pervasive and Mechanistically Diverse.Chemical reviews · 2017Review
- Antibacterial Activities of Bacteria Isolated from the Marine Sponges Isodictya compressa and Higginsia bidentifera Collected from Algoa Bay, South Africa.Marine drugs · 2017Article
- Comparative Metagenomics Reveals the Distinctive Adaptive Features of theFrontiers in microbiology · 2017Article
- Discovery of the New Plant Growth-Regulating Compound LYXLF2 Based on Manipulating the Halogenase in Amycolatopsis orientalis.Current microbiology · 2016Article
- Discovery Strategies of Bioactive Compounds Synthesized by Nonribosomal Peptide Synthetases and Type-I Polyketide Synthases Derived from Marine Microbiomes.Marine drugs · 2016Review
- Unusual flavoenzyme catalysis in marine bacteria.Current opinion in chemical biology · 2016Review
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4 authors.
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Abstract
Many marine sponges (Porifera) are known to contain large amounts of phylogenetically diverse microorganisms. Sponges are also known for their large arsenal of natural products, many of which are halogenated. In this study, 36 different FADH₂-dependent halogenase gene fragments were amplified from various Caribbean and Mediterranean sponges using newly designed degenerate PCR primers. Four unique halogenase-positive fosmid clones, all containing the highly conserved amino acid motif "GxGxxG", were identified in the microbial metagenome of Aplysina aerophoba. Sequence analysis of one halogenase-bearing fosmid revealed notably two open reading frames with high homologies to efflux and multidrug resistance proteins. Single cell genomic analysis allowed for a taxonomic assignment of the halogenase genes to specific symbiotic lineages. Specifically, the halogenase cluster S1 is predicted to be produced by a deltaproteobacterial symbiont and halogenase cluster S2 by a poribacterial sponge symbiont. An additional halogenase gene is possibly produced by an actinobacterial symbiont of marine sponges. The identification of three novel, phylogenetically, and possibly also functionally distinct halogenase gene clusters indicates that the microbial consortia of sponges are a valuable resource for novel enzymes involved in halogenation reactions.
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